RNA extraction

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==General Information==
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This is a general protocol page for extracting RNA from samples.
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==General Procedures==
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==Specific Protocols==
==Specific Protocols==
*[[Endy:RNA extraction]]
*[[Endy:RNA extraction]]
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==Notes==
==Notes==
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Some general notes on isolating RNA.
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These notes are focused on bacterial cells but please contribute to include information about plant, mammalian and other cells etc.
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#Centrifugation of cells can induce a stress response in cells altering transcript levels (potentially unequally across different transcripts).
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#[http://www1.qiagen.com/literature/handbooks/literature.aspx?id=1000278 "RNA''protect''"] is a reagent used to stabilize RNA content in bacterial cells grown in liquid culture.
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#*Works best if cells are grown in minimal media.  Works worst in LB liquid culture due to complex components.
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==Related information==
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See [[RNA]].
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==Reference==
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<biblio>
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#MeasuringGeneExpression isbn=0415374723
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</biblio>
[[Category:Protocol]]
[[Category:Protocol]]
[[Category:RNA]]
[[Category:RNA]]
[[Category:In vitro]]
[[Category:In vitro]]

Revision as of 12:34, 9 October 2007

This is a general protocol page for extracting RNA from samples.

Contents

Specific Protocols

Notes

Some general notes on isolating RNA.

These notes are focused on bacterial cells but please contribute to include information about plant, mammalian and other cells etc.

  1. Centrifugation of cells can induce a stress response in cells altering transcript levels (potentially unequally across different transcripts).
  2. "RNAprotect" is a reagent used to stabilize RNA content in bacterial cells grown in liquid culture.
    • Works best if cells are grown in minimal media. Works worst in LB liquid culture due to complex components.

Related information

See RNA.

Reference

  1. Matthew B. Avison. Measuring gene expression. New York, NY: Taylor & Francis Group, 2007. isbn:0415374723. [MeasuringGeneExpression]
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