RNA Quality Control - Revision history

Jakob Suckale: /* RNA Image galleries */ added example mouse total RNA electropherograms

2011-05-05T09:52:27Z

RNA Image galleries: added example mouse total RNA electropherograms

←Older revision		Revision as of 09:52, 5 May 2011
Line 18:		Line 18:
	<gallery>		<gallery>
	Image:Human RNA good quality.jpg\|Human RNA good quality		Image:Human RNA good quality.jpg\|Human RNA good quality
		+	Image:Mouse total RNA undegraded.PNG\|Mouse total RNA undegraded, RIN 10
		+	Image:Mouse total RNA slightly degraded.PNG\|Mouse total RNA slightly degraded, RIN 9.4
	Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality		Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality
	Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA		Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA

Jakob Suckale: /* Checking RNA/cRNA samples on an Agilent Bioanalyzer */ removed lost images from gallery

2011-05-05T09:50:21Z

Checking RNA/cRNA samples on an Agilent Bioanalyzer: removed lost images from gallery

←Older revision		Revision as of 09:50, 5 May 2011
Line 18:		Line 18:
	<gallery>		<gallery>
	Image:Human RNA good quality.jpg\|Human RNA good quality		Image:Human RNA good quality.jpg\|Human RNA good quality
-	~~Image:Human RNA slightly degraded.jpg\|Human RNA slightly degraded~~
-	~~Image:Human RNA very degraded.jpg\|Human RNA very degraded~~
-	~~Image:Human RNA brain.jpg\|Human RNA brain~~
-	~~Image:Human RNA muscle.jpg\|Human RNA muscle~~
-	~~Image:Human RNA blood.jpg\|Human RNA blood~~
-	~~Image:Drosphila RNA good quality.jpg\|Drosphila RNA good quality~~
	Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality		Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality
-	~~Image:Arabidopsis RNA slightly degraded.jpg\|Arabidopsis RNA slightly degraded~~
	Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA		Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA
	Image:Barley RNA good quality.jpg\|Barley RNA good quality		Image:Barley RNA good quality.jpg\|Barley RNA good quality
	Image:Wheat RNA good quality.jpg\|Wheat RNA good quality		Image:Wheat RNA good quality.jpg\|Wheat RNA good quality
-	~~Explanation of electropherograms.~~
	</gallery>		</gallery>
	</div>		</div>
Line 46:		Line 38:
	Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality		Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality
	Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality		Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality
-	~~Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting~~
-	~~Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting~~
-	~~Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting~~
-	~~Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting<br>~~
-	~~Explanation of electropherograms.~~
	</gallery>		</gallery>
	</div>		</div>

Jakob Suckale at 13:32, 4 August 2008

2008-08-04T13:32:25Z

←Older revision		Revision as of 13:32, 4 August 2008
Line 9:		Line 9:
	*For Affymetrix labeling reactiosns; concentrations of cRNA are generally around 1000-1500ng/µl for a 40µl IVT reaction using 20µl of cDNA template.		*For Affymetrix labeling reactiosns; concentrations of cRNA are generally around 1000-1500ng/µl for a 40µl IVT reaction using 20µl of cDNA template.

-	==Checking RNA/cRNA samples on an ~~agilent bioanalyzer~~==	+	==Checking RNA/cRNA samples on an Agilent [[Bioanalyzer]]==
	Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA. The bioanalyzer software produces a RIN (RNA integrity number) this gives a measure of sample quality that is not subjective. Howver it needs to be used with some caution and is not as useful as it sounds.		Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA. The bioanalyzer software produces a RIN (RNA integrity number) this gives a measure of sample quality that is not subjective. Howver it needs to be used with some caution and is not as useful as it sounds.

Reshma P. Shetty at 16:39, 9 May 2007

2007-05-09T16:39:55Z

←Older revision		Revision as of 16:39, 9 May 2007
Line 60:		Line 60:
	*mail to jamesdothadfieldatcancerdotorgdotuk		*mail to jamesdothadfieldatcancerdotorgdotuk
	*This page was created by James Hadfield on 12 October 2006. I hope you found it useful.		*This page was created by James Hadfield on 12 October 2006. I hope you found it useful.
		+
		+	[[Category:Protocol]]
		+	[[Category:In vitro]]
		+	[[Category:RNA]]

Jamesh008 at 11:35, 3 November 2006

2006-11-03T11:35:31Z

←Older revision		Revision as of 11:35, 3 November 2006
Line 38:		Line 38:
	cRNA electropherograms have different profiles depending on species and starting material quality.		cRNA electropherograms have different profiles depending on species and starting material quality.
	<gallery>		<gallery>
-	~~Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality~~	+
-	~~Image:Affymetrix one cycle Arabidopsis cRNA good quality.jpg\|Affymetrix one cycle Arabidopsis cRNA good quality~~	+
	Image:Affymetrix One cycle Human cRNA good quality.jpg\|Affymetrix One cycle Human cRNA good quality		Image:Affymetrix One cycle Human cRNA good quality.jpg\|Affymetrix One cycle Human cRNA good quality
	Image:Affymetrix Two cycle Human cRNA good quality.jpg\|Affymetrix Two cycle Human cRNA good quality		Image:Affymetrix Two cycle Human cRNA good quality.jpg\|Affymetrix Two cycle Human cRNA good quality
		+	Image:Affymetrix one cycle Arabidopsis cRNA good quality.jpg\|Affymetrix one cycle Arabidopsis cRNA good quality
		+	Image:Affymetrix one cycle Arabidopsis single cell aRNA cRNA.jpg\|Affymetrix one cycle Arabidopsis single cell aRNA cRNA
	Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality		Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality
		+	Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality
	Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality		Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality
-	~~Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality~~
-	~~Image:Affymetrix one cycle Arabidopsis single cell aRNA cRNA.jpg\|Affymetrix one cycle Arabidopsis single cell aRNA cRNA~~
	Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting		Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting
	Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting		Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting
	Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting		Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting
-	Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting	+	Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting<br>
	Explanation of electropherograms.		Explanation of electropherograms.
	</gallery>		</gallery>

Jamesh008: editing of galleries

2006-11-03T11:28:06Z

editing of galleries

←Older revision		Revision as of 11:28, 3 November 2006
Line 10:		Line 10:

	==Checking RNA/cRNA samples on an agilent bioanalyzer==		==Checking RNA/cRNA samples on an agilent bioanalyzer==
-	Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA.	+	Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA. The bioanalyzer software produces a RIN (RNA integrity number) this gives a measure of sample quality that is not subjective. Howver it needs to be used with some caution and is not as useful as it sounds.

	===RNA Image galleries===		===RNA Image galleries===
		+	<div style="padding: 10px; width: 720px; border: 2px solid #000000;">
	The following are examples of typical Human RNA examined on an Agilent 2100 BioAnalyser.		The following are examples of typical Human RNA examined on an Agilent 2100 BioAnalyser.
	RNA electropherograms have different profiles depending on species, tissue and starting material quality.		RNA electropherograms have different profiles depending on species, tissue and starting material quality.
-
	<gallery>		<gallery>
-	Image:~~HumanRNA~~.~~png~~	+	Image:Human RNA good quality.jpg\|Human RNA good quality
-	~~Image:Image name\|thumb~~\|Human	+	Image:Human RNA slightly degraded.jpg\|Human RNA slightly degraded
-	Image:~~Image name\|thumb~~\|Human slightly degraded	+	Image:Human RNA very degraded.jpg\|Human RNA very degraded
-	Image:~~Image name\|thumb~~\|Human very degraded	+	Image:Human RNA brain.jpg\|Human RNA brain
-	Image:~~Image name\|thumb\|Arabidopsis~~	+	Image:Human RNA muscle.jpg\|Human RNA muscle
-	~~Image:Image name\|thumb~~\|Human brain	+	Image:Human RNA blood.jpg\|Human RNA blood
-	Image:~~Image name\|thumb~~\|Human muscle	+	Image:Drosphila RNA good quality.jpg\|Drosphila RNA good quality
-	Image:~~Image name\|thumb~~\|Human blood	+	Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality
-	Image:~~Image name\|thumb~~\|Drosphila	+	Image:Arabidopsis RNA slightly degraded.jpg\|Arabidopsis RNA slightly degraded
-	Image:Image ~~name~~\|~~thumb\|Drosphila~~ degraded	+	Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA
-	Image:~~Image name\|thumb~~\|Arabidopsis	+	Image:Barley RNA good quality.jpg\|Barley RNA good quality
-	Image:Image ~~name~~\|~~thumb\|Arabidopsis degraded~~	+	Image:Wheat RNA good quality.jpg\|Wheat RNA good quality
		+	Explanation of electropherograms.
	</gallery>		</gallery>
-	~~Explanation of electropherograms.~~	+	</div>

	===cRNA Image galleries===		===cRNA Image galleries===
		+	<div style="padding: 10px; width: 720px; border: 2px solid #000000;">
	The following are examples of typical cRNA products examined on an Agilent 2100 BioAnalyser.		The following are examples of typical cRNA products examined on an Agilent 2100 BioAnalyser.
	cRNA electropherograms have different profiles depending on species and starting material quality.		cRNA electropherograms have different profiles depending on species and starting material quality.
-
	<gallery>		<gallery>
-	Image:Image ~~name~~\|~~thumb~~\|Human cRNA ~~10ug starting RNA~~	+	Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality
-		+	Image:Affymetrix one cycle Arabidopsis cRNA good quality.jpg\|Affymetrix one cycle Arabidopsis cRNA good quality
-	Image:Image ~~name~~\|~~thumb~~\|~~Human~~ cRNA ~~5ug starting RNA~~	+	Image:Affymetrix One cycle Human cRNA good quality.jpg\|Affymetrix One cycle Human cRNA good quality
-	Image:~~Image name~~\|~~thumb~~\|Human cRNA ~~1ug~~ starting ~~RNA~~	+	Image:Affymetrix Two cycle Human cRNA good quality.jpg\|Affymetrix Two cycle Human cRNA good quality
-	Image:~~Image name\|thumb~~\|Human cRNA ~~100ng~~ starting ~~RNA~~	+	Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality
-	Image:Image ~~name\|thumb~~\|Human cRNA ~~10ng~~ starting ~~RNA~~	+	Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality
		+	Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality
		+	Image:Affymetrix one cycle Arabidopsis single cell aRNA cRNA.jpg\|Affymetrix one cycle Arabidopsis single cell aRNA cRNA
		+	Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting
		+	Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting
		+	Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting
		+	Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting
		+	Explanation of electropherograms.
	</gallery>		</gallery>
-	~~Explanation of electropherograms.~~	+	</div>

	==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==		==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==

Jamesh008: add contact info

2006-10-13T08:03:16Z

add contact info

←Older revision		Revision as of 08:03, 13 October 2006
Line 48:		Line 48:
	==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==		==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==
	I still need to add a formaldhyde gel protocol, anyone else got one?		I still need to add a formaldhyde gel protocol, anyone else got one?
		+
		+	==Contact==
		+	*mail to jamesdothadfieldatcancerdotorgdotuk
		+	*This page was created by James Hadfield on 12 October 2006. I hope you found it useful.

Jamesh008 at 13:11, 12 October 2006

2006-10-12T13:11:46Z

New page

== Quality control of RNA==

RNA Quality control
This protocol will assist you in performing RNA quality control for a variet of purposes; Microarray, real-time and Northern analysis.

==Checking RNA/cRNA samples on a nanodrop Spec==
*Check concentration, A260/A280, A260/A230 on [http://www.nanodrop.com/ Nanodrop] Spectrophotometer.
*Maintain the A260/A280 ratio close to 2.0 for pure RNA (ratios between 1.9 and 2.1 are acceptable).
*For Affymetrix labeling reactiosns; concentrations of cRNA are generally around 1000-1500ng/µl for a 40µl IVT reaction using 20µl of cDNA template.

==Checking RNA/cRNA samples on an agilent bioanalyzer==
Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA.

===RNA Image galleries===
The following are examples of typical Human RNA examined on an Agilent 2100 BioAnalyser.
RNA electropherograms have different profiles depending on species, tissue and starting material quality.

<gallery>
Image:HumanRNA.png
Image:Image name|thumb|Human
Image:Image name|thumb|Human slightly degraded
Image:Image name|thumb|Human very degraded
Image:Image name|thumb|Arabidopsis
Image:Image name|thumb|Human brain
Image:Image name|thumb|Human muscle
Image:Image name|thumb|Human blood
Image:Image name|thumb|Drosphila
Image:Image name|thumb|Drosphila degraded
Image:Image name|thumb|Arabidopsis
Image:Image name|thumb|Arabidopsis degraded
</gallery>
Explanation of electropherograms.

===cRNA Image galleries===
The following are examples of typical cRNA products examined on an Agilent 2100 BioAnalyser.
cRNA electropherograms have different profiles depending on species and starting material quality.

<gallery>
Image:Image name|thumb|Human cRNA 10ug starting RNA

Image:Image name|thumb|Human cRNA 5ug starting RNA
Image:Image name|thumb|Human cRNA 1ug starting RNA
Image:Image name|thumb|Human cRNA 100ng starting RNA
Image:Image name|thumb|Human cRNA 10ng starting RNA
</gallery>
Explanation of electropherograms.

==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==
I still need to add a formaldhyde gel protocol, anyone else got one?

←Older revision		Revision as of 09:52, 5 May 2011
Line 18:		Line 18:
	<gallery>		<gallery>
	Image:Human RNA good quality.jpg\|Human RNA good quality		Image:Human RNA good quality.jpg\|Human RNA good quality
		+	Image:Mouse total RNA undegraded.PNG\|Mouse total RNA undegraded, RIN 10
		+	Image:Mouse total RNA slightly degraded.PNG\|Mouse total RNA slightly degraded, RIN 9.4
	Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality		Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality
	Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA		Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA

←Older revision		Revision as of 09:50, 5 May 2011
Line 18:		Line 18:
	<gallery>		<gallery>
	Image:Human RNA good quality.jpg\|Human RNA good quality		Image:Human RNA good quality.jpg\|Human RNA good quality
-	~~Image:Human RNA slightly degraded.jpg\|Human RNA slightly degraded~~
-	~~Image:Human RNA very degraded.jpg\|Human RNA very degraded~~
-	~~Image:Human RNA brain.jpg\|Human RNA brain~~
-	~~Image:Human RNA muscle.jpg\|Human RNA muscle~~
-	~~Image:Human RNA blood.jpg\|Human RNA blood~~
-	~~Image:Drosphila RNA good quality.jpg\|Drosphila RNA good quality~~
	Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality		Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality
-	~~Image:Arabidopsis RNA slightly degraded.jpg\|Arabidopsis RNA slightly degraded~~
	Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA		Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA
	Image:Barley RNA good quality.jpg\|Barley RNA good quality		Image:Barley RNA good quality.jpg\|Barley RNA good quality
	Image:Wheat RNA good quality.jpg\|Wheat RNA good quality		Image:Wheat RNA good quality.jpg\|Wheat RNA good quality
-	~~Explanation of electropherograms.~~
	</gallery>		</gallery>
	</div>		</div>
Line 46:		Line 38:
	Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality		Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality
	Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality		Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality
-	~~Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting~~
-	~~Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting~~
-	~~Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting~~
-	~~Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting<br>~~
-	~~Explanation of electropherograms.~~
	</gallery>		</gallery>
	</div>		</div>

←Older revision		Revision as of 11:35, 3 November 2006
Line 38:		Line 38:
	cRNA electropherograms have different profiles depending on species and starting material quality.		cRNA electropherograms have different profiles depending on species and starting material quality.
	<gallery>		<gallery>
-	~~Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality~~	+
-	~~Image:Affymetrix one cycle Arabidopsis cRNA good quality.jpg\|Affymetrix one cycle Arabidopsis cRNA good quality~~	+
	Image:Affymetrix One cycle Human cRNA good quality.jpg\|Affymetrix One cycle Human cRNA good quality		Image:Affymetrix One cycle Human cRNA good quality.jpg\|Affymetrix One cycle Human cRNA good quality
	Image:Affymetrix Two cycle Human cRNA good quality.jpg\|Affymetrix Two cycle Human cRNA good quality		Image:Affymetrix Two cycle Human cRNA good quality.jpg\|Affymetrix Two cycle Human cRNA good quality
		+	Image:Affymetrix one cycle Arabidopsis cRNA good quality.jpg\|Affymetrix one cycle Arabidopsis cRNA good quality
		+	Image:Affymetrix one cycle Arabidopsis single cell aRNA cRNA.jpg\|Affymetrix one cycle Arabidopsis single cell aRNA cRNA
	Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality		Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality
		+	Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality
	Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality		Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality
-	~~Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality~~
-	~~Image:Affymetrix one cycle Arabidopsis single cell aRNA cRNA.jpg\|Affymetrix one cycle Arabidopsis single cell aRNA cRNA~~
	Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting		Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting
	Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting		Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting
	Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting		Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting
-	Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting	+	Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting<br>
	Explanation of electropherograms.		Explanation of electropherograms.
	</gallery>		</gallery>

←Older revision		Revision as of 11:28, 3 November 2006
Line 10:		Line 10:

	==Checking RNA/cRNA samples on an agilent bioanalyzer==		==Checking RNA/cRNA samples on an agilent bioanalyzer==
-	Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA.	+	Agilent [http://www.chem.agilent.com/Scripts/PDS.asp?lPage=51 BioAnalyzer] Gel electrophoresis of the RNA is done to estimate the quality of the RNA. The bioanalyzer software produces a RIN (RNA integrity number) this gives a measure of sample quality that is not subjective. Howver it needs to be used with some caution and is not as useful as it sounds.

	===RNA Image galleries===		===RNA Image galleries===
		+	<div style="padding: 10px; width: 720px; border: 2px solid #000000;">
	The following are examples of typical Human RNA examined on an Agilent 2100 BioAnalyser.		The following are examples of typical Human RNA examined on an Agilent 2100 BioAnalyser.
	RNA electropherograms have different profiles depending on species, tissue and starting material quality.		RNA electropherograms have different profiles depending on species, tissue and starting material quality.
-
	<gallery>		<gallery>
-	Image:~~HumanRNA~~.~~png~~	+	Image:Human RNA good quality.jpg\|Human RNA good quality
-	~~Image:Image name\|thumb~~\|Human	+	Image:Human RNA slightly degraded.jpg\|Human RNA slightly degraded
-	Image:~~Image name\|thumb~~\|Human slightly degraded	+	Image:Human RNA very degraded.jpg\|Human RNA very degraded
-	Image:~~Image name\|thumb~~\|Human very degraded	+	Image:Human RNA brain.jpg\|Human RNA brain
-	Image:~~Image name\|thumb\|Arabidopsis~~	+	Image:Human RNA muscle.jpg\|Human RNA muscle
-	~~Image:Image name\|thumb~~\|Human brain	+	Image:Human RNA blood.jpg\|Human RNA blood
-	Image:~~Image name\|thumb~~\|Human muscle	+	Image:Drosphila RNA good quality.jpg\|Drosphila RNA good quality
-	Image:~~Image name\|thumb~~\|Human blood	+	Image:Arabidopsis RNA good quality.jpg\|Arabidopsis RNA good quality
-	Image:~~Image name\|thumb~~\|Drosphila	+	Image:Arabidopsis RNA slightly degraded.jpg\|Arabidopsis RNA slightly degraded
-	Image:Image ~~name~~\|~~thumb\|Drosphila~~ degraded	+	Image:Arabidopsis single cell aRNA.jpg\|Arabidopsis single cell aRNA
-	Image:~~Image name\|thumb~~\|Arabidopsis	+	Image:Barley RNA good quality.jpg\|Barley RNA good quality
-	Image:Image ~~name~~\|~~thumb\|Arabidopsis degraded~~	+	Image:Wheat RNA good quality.jpg\|Wheat RNA good quality
		+	Explanation of electropherograms.
	</gallery>		</gallery>
-	~~Explanation of electropherograms.~~	+	</div>

	===cRNA Image galleries===		===cRNA Image galleries===
		+	<div style="padding: 10px; width: 720px; border: 2px solid #000000;">
	The following are examples of typical cRNA products examined on an Agilent 2100 BioAnalyser.		The following are examples of typical cRNA products examined on an Agilent 2100 BioAnalyser.
	cRNA electropherograms have different profiles depending on species and starting material quality.		cRNA electropherograms have different profiles depending on species and starting material quality.
-
	<gallery>		<gallery>
-	Image:Image ~~name~~\|~~thumb~~\|Human cRNA ~~10ug starting RNA~~	+	Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality
-		+	Image:Affymetrix one cycle Arabidopsis cRNA good quality.jpg\|Affymetrix one cycle Arabidopsis cRNA good quality
-	Image:Image ~~name~~\|~~thumb~~\|~~Human~~ cRNA ~~5ug starting RNA~~	+	Image:Affymetrix One cycle Human cRNA good quality.jpg\|Affymetrix One cycle Human cRNA good quality
-	Image:~~Image name~~\|~~thumb~~\|Human cRNA ~~1ug~~ starting ~~RNA~~	+	Image:Affymetrix Two cycle Human cRNA good quality.jpg\|Affymetrix Two cycle Human cRNA good quality
-	Image:~~Image name\|thumb~~\|Human cRNA ~~100ng~~ starting ~~RNA~~	+	Image:Affymetrix one cycle Barley cRNA good quality.jpg\|Affymetrix one cycle Barley cRNA good quality
-	Image:Image ~~name\|thumb~~\|Human cRNA ~~10ng~~ starting ~~RNA~~	+	Image:Affymetrix one cycle Wheat cRNA poor quality.jpg\|Affymetrix one cycle Wheat cRNA poor quality
		+	Image:Affymetrix one cycle Wheat cRNA good quality.jpg\|Affymetrix one cycle Wheat cRNA good quality
		+	Image:Affymetrix one cycle Arabidopsis single cell aRNA cRNA.jpg\|Affymetrix one cycle Arabidopsis single cell aRNA cRNA
		+	Image:Affymetrix Two cycle Human cRNA 100ng starting.jpg\|Affymetrix Two cycle Human cRNA 100ng starting
		+	Image:Affymetrix Two cycle Human cRNA 10ng starting.jpg\|Affymetrix Two cycle Human cRNA 10ng starting
		+	Image:Affymetrix Two cycle Human cRNA 5ng starting.jpg\|Affymetrix Two cycle Human cRNA 5ng starting
		+	Image:Affymetrix Two cycle Human cRNA 1ng starting.jpg\|Affymetrix Two cycle Human cRNA 1ng starting
		+	Explanation of electropherograms.
	</gallery>		</gallery>
-	~~Explanation of electropherograms.~~	+	</div>

	==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==		==Checking RNA/cRNA Samples on a denaturing formaldehyde gel==