Quint Lab:yeast western blot

From OpenWetWare

Revision as of 07:42, 17 October 2008 by Marcel Quint (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

home        research        people        papers        teaching        join us        internal        pictures        contact       


western blot yeast

  1. spin down the over night culture 2 min 10000 rpm
  2. pour off the SN
  3. add 2x Lämmli: 50 – 100 µl → vortex
  4. boil for 5 min → chill on ice
  5. spin down 30 s top speed→ chill on ice
  6. 15 µl on gel
  7. 2 ml over night culture (selective medium) @ 30°C


pGBKT7: α-c-myc (1:5000)
pGADT7: α-HA (1:5000)
pJG4-5/pJCL: α-HA (1:5000)
pEG202: α-LexA

Personal tools