Quint Lab:preparing electrocompetent A.rhizogenes cells + electroporation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Marcel Quint (talk | contribs) |
Marcel Quint (talk | contribs) No edit summary |
||
Line 1: | Line 1: | ||
{{Template:Quint Lab}} | |||
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;" | |||
|-valign="top" | |||
|width=300px style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" | | |||
==preparing electrocompetent ''A. rhizogenis'' cells== | ==preparing electrocompetent ''A. rhizogenis'' cells== | ||
#Inoculate 5ml overnight culture (LB medium + strep) with ''A. rhizogenis''. Grow at 28°C (180 rpm) to an OD = 1 (takes ~ 12 hours). | #Inoculate 5ml overnight culture (LB medium + strep) with ''A. rhizogenis''. Grow at 28°C (180 rpm) to an OD = 1 (takes ~ 12 hours). |
Latest revision as of 07:03, 16 October 2008
preparing electrocompetent A. rhizogenis cells
elctroporation
|