Quint Lab:preparing electrocompetent A.rhizogenes cells + electroporation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: ==preparing electrocompetent A.rhizogenis cells== #Inoculate 5ml overnight culture (LB medium + strep) with A. rhizogenis. Grow at 28°C (180 rpm) to an OD = 1 (takes ~ 12 hours). #Transfe...) |
Marcel Quint (talk | contribs) No edit summary |
||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
==preparing electrocompetent A.rhizogenis cells== | {{Template:Quint Lab}} | ||
#Inoculate 5ml overnight culture (LB medium + strep) with A. rhizogenis. Grow at 28°C (180 rpm) to an OD = 1 (takes ~ 12 hours). | {| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;" | ||
|-valign="top" | |||
|width=300px style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" | | |||
==preparing electrocompetent ''A. rhizogenis'' cells== | |||
#Inoculate 5ml overnight culture (LB medium + strep) with ''A. rhizogenis''. Grow at 28°C (180 rpm) to an OD = 1 (takes ~ 12 hours). | |||
#Transfer ON culture into 200 ml preheated LB medium (+ strep). Allow cells to grow at 28°C (180 rpm) to an OD 0,5-0,6 (takes at least 4-5-h). | #Transfer ON culture into 200 ml preheated LB medium (+ strep). Allow cells to grow at 28°C (180 rpm) to an OD 0,5-0,6 (takes at least 4-5-h). | ||
#Put cell culture on ice for 15 min. | #Put cell culture on ice for 15 min. |
Latest revision as of 07:03, 16 October 2008
preparing electrocompetent A. rhizogenis cells
elctroporation
|