Quint Lab:kanamycin selection of transgenic seeds

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kanamycin selection of transgenic seeds

Selection of transformed Arabidopsis (Kanamycin Selection)

  • Sterilize seeds and stratify them for 2 d at 4°C in the dark
  • Sow seeds on ATS + 50 μg/ml Kanamycin
  • Cultivate seedlings for 10 d at 20°C under LD conditions


Evaluation:

  • transformed seedlings are resistant to Kanamycin and show green and open, expanded cotyledons
  • untransformed seedlings are sensitive to Kanamycin and therefore cotyledons appear yellow and closed


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