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resources

• primer lists
  
• enzyme lists
  
• seeds
  
• ordering
  

papers/lab meetings/seminars papers of the month lab meeting/journal club ipb progress reports/seminars SFB648 seminars

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online tools dna/protein analysis: sequence converter dna clean-up protein clean-up reverse complement translate dna predict protein mw protein motif scan restriction analysis genetic code amino acid structures plasmapper oligos: oligo analysis primer3 duplex analysis blasts/alignments: pairwise dna alignment protein alignment blast alignment clustalw - multiple alignments blastn blastp Vicogenta - comparing genomes to arabidopsis arabidopsis tools: tair nasc plants-ubq salk t-dnas genevestigator efp-browser/expression angler/etc. GEB - gene expression browser for arabidopsis tiling array expression atlas arex mapman web mirna designer tile viz tiling array express 1001 genomes POLYMORPH reseq SNPs arabidopsis hormone database JIC microsatellite mapping markers Vicogenta - comparing genomes to arabidopsis medicago tools: medicago gene expression atlas medicago database natural variation/polymorphisms: msqt - multiple snp query tool masc snp database caps finder polymorph - reseq. snps atprimer ecotypes JIC microsatellite mapping markers calculators: vector:insert ratios molar conversions - moles ↔ weight concentration conversions - molar units ↔ weight/volume pcr mastermix calculator buffer calculator pcr mastermix calculator

white board The Large Hadron Rap medicago gene expression atlas the postdoc interview craig venter's grand vision on synthetic genomics → video Kits and their unique role in molecular biology: a brief retrospective recycling electroporation cuvettes pimp your plasmid growth medium buffer calculator Control Your Lab Computers From Home run faster gels → new electrophoresis buffers how-to-become-a-world-expert-in-your-field → artikel quick and dirty screening for cloned inserts awesome video → cellular visions: the inner life of a cell

lab protocols → check also openwetware protocols e. coli cacl2-competent cells e.coli e.coli transformation chemical electrocompetent cells e.coli + electroporation colony pcr agros electrocompetent A.rhizogenes cells + electroporation agrobacterium plasmid miniprep arabidopsis arabidopsis dna isolation - quick and dirty arabidopsis transformation arabidopsis protoplast transformation transient transformation and expression kanamycin selection of transgenic seeds ems mutagenesis ats plates iaa extraction and quantification yeast → for general yeast handling follow Clontech Yeast Protocols Handbook → for Y2H follow Clontech Matchmaker Library Construction & Screening Kit genomic dna non-lethal ß-galactosidase assay Short version ß-galactosidase assay plasmid prep yeast transformation yeast western blot mating in 24-well plates yeast media general ligation (sticky ends) northern western