Quint Lab:Back Door

resources

• primer lists
  
• enzyme lists
  
• seeds
  
• ordering
  

papers/lab meetings/seminars

• papers of the month
  
• lab meeting/journal club
• ipb progress reports/seminars
• SFB648 seminars

online tools

dna/protein analysis:

• sequence converter
• dna clean-up
• protein clean-up
• reverse complement
• translate dna
• predict protein mw
• protein motif scan
• restriction analysis
  
• genetic code
  
• amino acid structures
  
• plasmapper

oligos:

• oligo analysis
• primer3
• duplex analysis

blasts/alignments:

• pairwise dna alignment
• protein alignment
• blast alignment
• clustalw - multiple alignments
• blastn
• blastp

arabidopsis tools:

• tair
• nasc
• plants-ubq
• salk t-dnas
• genevestigator
• efp-browser/expression angler/etc.
• tiling array expression atlas
• arex
• mapman
• web mirna designer

medicago tools:

• medicago gene expression atlas
• medicago database

natural variation/polymorphisms:

• msqt - multiple snp query tool
• masc snp database
• caps finder
• polymorph - reseq. snps
• atprimer ecotypes

calculators:

• vector:insert ratios
• molar conversions - moles ↔ weight
• concentration conversions - molar units ↔ weight/volume
• pcr mastermix calculator
• buffer calculator
• pcr mastermix calculator

white board

• The Large Hadron Rap
• medicago gene expression atlas
• the postdoc interview
• craig venter's grand vision on synthetic genomics → video
• Kits and their unique role in molecular biology: a brief retrospective
• recycling electroporation cuvettes
• pimp your plasmid growth medium
• buffer calculator
• Control Your Lab Computers From Home
• run faster gels → new electrophoresis buffers
• how-to-become-a-world-expert-in-your-field → artikel
• quick and dirty screening for cloned inserts
• awesome video → cellular visions: the inner life of a cell

lab protocols

→ check also openwetware protocols

e. coli

• cacl2-competent cells e.coli
• e.coli transformation chemical
• electrocompetent cells e.coli + electroporation
• colony pcr

agros

• electrocompetent A.rhizogenes cells + electroporation
• agrobacterium plasmid miniprep

arabidopsis

• arabidopsis dna isolation - quick and dirty
• arabidopsis transformation
• arabidopsis protoplast transformation
• transient transformation and expression
• kanamycin selection of transgenic seeds
• ems mutagenesis
• ats plates
• iaa extraction and quantification

yeast
→ for general yeast handling follow Yeast Protocols Handbook (Clontech; PT3024-1/ PR13103)
→ for Y2H follow Matchmaker Library Construction & Screening Kit (Clontech; PT3529/ PR13384)

• genomic dna
• non-lethal ß-galactosidase assay
• Short version ß-galactosidase assay
• plasmid prep
• yeast transformation
• yeast western blot
• mating in 24-well plates
• yeast media

general

• ligation (sticky ends)
• northern
• western