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resources

• primer lists
  
• enzyme lists
  
• seeds
  
• ordering
  

papers/lab meetings/seminars papers of the month lab meeting/journal club ipb progress reports/seminars SFB648 seminars

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online tools dna/protein analysis: sequence converter dna clean-up protein clean-up reverse complement translate dna predict protein mw protein motif scan restriction analysis genetic code amino acid structures plasmapper oligos: oligo analysis primer3 duplex analysis blasts/alignments: pairwise dna alignment protein alignment blast alignment clustalw - multiple alignments blastn blastp arabidopsis tools: tair nasc plants-ubq salk t-dnas genevestigator efp-browser/expression angler/etc. tiling array expression atlas arex mapman web mirna designer medicago tools: medicago gene expression atlas medicago database natural variation/polymorphisms: msqt - multiple snp query tool masc snp database caps finder polymorph - reseq. snps atprimer ecotypes calculators: vector:insert ratios molar conversions - moles ↔ weight concentration conversions - molar units ↔ weight/volume pcr mastermix calculator buffer calculator pcr mastermix calculator

white board The Large Hadron Rap medicago gene expression atlas the postdoc interview craig venter's grand vision on synthetic genomics → video Kits and their unique role in molecular biology: a brief retrospective recycling electroporation cuvettes pimp your plasmid growth medium buffer calculator Control Your Lab Computers From Home run faster gels → new electrophoresis buffers how-to-become-a-world-expert-in-your-field → artikel quick and dirty screening for cloned inserts awesome video → cellular visions: the inner life of a cell

lab protocols → check also openwetware protocols e. coli cacl2-competent cells e.coli e.coli transformation chemical electrocompetent cells e.coli + electroporation colony pcr agros electrocompetent A.rhizogenes cells + electroporation agrobacterium plasmid miniprep arabidopsis arabidopsis dna isolation - quick and dirty arabidopsis transformation arabidopsis protoplast transformation transient transformation and expression kanamycin selection of transgenic seeds ems mutagenesis ats plates iaa extraction and quantification yeast → for general yeast handling follow Yeast Protocols Handbook (Clontech; PT3024-1/ PR13103) → for Y2H follow Matchmaker Library Construction & Screening Kit (Clontech; PT3529/ PR13384) genomic dna non-lethal ß-galactosidase assay Short version ß-galactosidase assay plasmid prep yeast transformation yeast western blot mating in 24-well plates yeast media general ligation (sticky ends) northern western