Quint Lab:Back Door

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ressources

• primer lists
  
• enzyme lists
  
• seeds
  
• ordering
  

papers/lab meetings/seminars papers of the month lab meeting/journal club ipb progress reports/seminars SFB648 seminars

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online tools dna/protein analysis: sequence converter dna clean-up protein clean-up reverse complement translate dna predict protein mw protein motif scan restriction analysis genetic code amino acid structures plasmapper oligos: oligo analysis primer3 duplex analysis blasts/alignments: pairwise dna alignment protein alignment blast alignment clustalw - multiple alignments blastn blastp arabidopsis tools: tair nasc plants-ubq salk t-dnas genevestigator efp-browser/expression angler/etc. arex mapman web mirna designer natural variation/polymorphisms: msqt - multiple snp query tool masc snp database caps finder polymorph - reseq. snps atprimer ecotypes calculators: vector:insert ratios molar conversions - moles ↔ weight concentration conversions - molar units ↔ weight/volume pcr mastermix calculator buffer calculator pcr mastermix calculator

white board Pimp Your Plasmid Growth Medium buffer calculator http://bitesizebio.com/2008/04/14/lab-remote-desktop/ Control Your Lab Computers From Home] run faster gels → new electrophoresis buffers how-to-become-a-world-expert-in-your-field → artikel quick and dirty screening for cloned inserts awesome video → cellular visions: the inner life of a cell

lab protocols → check also openwetware protocols arabidopsis dna isolation - quick and dirty arabidopsis transformation ems mutagenesis colony pcr cacl2-competent cells e.coli electrocompetent cells e.coli + electroporation agrobacterium plasmid miniprep ats plates iaa extraction and quantification ligation (sticky ends) northern western e.coli transformation chemical electrocompetent A.rhizogenes cells + electroporation