Quantification of nucleic acids

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Nucleic acids are typically quantitated based on their absorbance of ultraviolet light. Each base has a different absorbance maximum, with the average being at 260 nm. Ambion has a good site that gives a variety of parameters for nucleotides and nucleic acids [1].

Quantification of RNA

RNA blotting and RPAs are the gold standards, since no amplification is involved, but require more RNA. Q-PCR is the most sensitive method and can discriminate closely related mRNAs. It is therefore suitable for quickly quantifying low amounts of RNA.

Quantification of DNA

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