http://openwetware.org/index.php?title=QRT-PCR/Two_tubes&feed=atom&action=history 2013-05-23T22:31:02Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=QRT-PCR/Two_tubes&diff=157628&oldid=prev 2007-10-10T23:56:54Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:56, 10 October 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Overview==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Overview==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The first tube produces cDNA from an RNA sample.&nbsp; The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules.&nbsp; The reaction is stopped by heating and an aliquot is taken to serve as the template for a sequence specific [[qPCR]] step.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The first tube produces cDNA from an RNA sample <ins class="diffchange diffchange-inline">&lt;cite&gt;MeasuringGeneExpression&lt;/cite&gt;</ins>.&nbsp; The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules.&nbsp; The reaction is stopped by heating and an aliquot is taken to serve as the template for a sequence specific [[qPCR]] step.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr> <!-- diff generator: internal 2013-05-23 22:31:02 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=QRT-PCR/Two_tubes&diff=157209&oldid=prev 2007-10-09T21:21:30Z

<p><a href="/wiki/QRT-PCR/Two_tube" class="mw-redirect" title="QRT-PCR/Two tube">QRT-PCR/Two tube</a> moved to <a href="/wiki/QRT-PCR/Two_tubes" title="QRT-PCR/Two tubes">QRT-PCR/Two tubes</a></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:21, 9 October 2007</td> </tr> <!-- diff generator: internal 2013-05-19 04:01:10 --> <!-- diff cache key owwdb:diff:version:1.11a:oldid:157201:newid:157209 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=QRT-PCR/Two_tubes&diff=157201&oldid=prev 2007-10-09T21:15:15Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:15, 9 October 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;/biblio&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;/biblio&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category:Protocol]] [[Category:In vitro<del class="diffchange diffchange-inline">]] [[Category:DNA</del>]] [[Category:RNA]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category:Protocol]] [[Category:In vitro]] [[Category:RNA]]</div></td></tr> <!-- diff generator: internal 2013-05-23 22:31:02 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=QRT-PCR/Two_tubes&diff=157176&oldid=prev 2007-10-09T20:15:10Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:15, 9 October 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The first tube produces cDNA from an RNA sample.&nbsp; The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Overview==</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The first tube produces cDNA from an RNA sample.&nbsp; The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules. <ins class="diffchange diffchange-inline"> </ins>The <ins class="diffchange diffchange-inline">reaction </ins>is <ins class="diffchange diffchange-inline">stopped by heating and an aliquot is taken to serve as the template for a </ins>sequence specific [[qPCR]] step.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The <del class="diffchange diffchange-inline">second tube </del>is sequence specific [[qPCR]] step.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr> <!-- diff generator: internal 2013-05-23 22:31:02 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=QRT-PCR/Two_tubes&diff=157173&oldid=prev 2007-10-09T20:12:32Z

<p>New page: The first tube produces cDNA from an RNA sample. The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of...</p> <p><b>New page</b></p><div>The first tube produces cDNA from an RNA sample. The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules.<br /> <br /> The second tube is sequence specific [[qPCR]] step.<br /> <br /> ==References==<br /> &lt;biblio&gt;<br /> #MeasuringGeneExpression isbn=0415374723<br /> &lt;/biblio&gt;<br /> <br /> [[Category:Protocol]] [[Category:In vitro]] [[Category:DNA]] [[Category:RNA]]</div>

Reshma P. Shetty