QRT-PCR/Single tube

From OpenWetWare

< QRT-PCR
Revision as of 16:10, 26 March 2007 by Btarlow (Talk | contribs)
Jump to: navigation, search

This protocol describes one-step real time quantitivie PCR to quantify relative levels of RNA in

Contents

Starting Materials

  • Validated PCR primers that are efficient over the range of RNA that you are assaying.

Basic Principle

  • RNA is reverse transcribed in a single well.
  • PCR reaction procedes. The levels of template are quantified during each cycle.

Protocol

Analysis

Delta-Delta Ct Method. See Livak KJ, Schmittgen. Methods 25 402-408 (2001)

Personal tools