Q-PCR - Revision history

Torsten Waldminghaus at 17:29, 23 February 2009

Torsten Waldminghaus — Mon, 23 Feb 2009 17:29:14 GMT

←Older revision		Revision as of 17:29, 23 February 2009
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		+	{{back to protocols}}
	{{dablink\|[[QRT-PCR\|quantitative reverse transcription PCR]]}}		{{dablink\|[[QRT-PCR\|quantitative reverse transcription PCR]]}}

Jakob Suckale: /* See also */ some links moved to QRT-PCR

Jakob Suckale — Mon, 14 Jan 2008 17:45:18 GMT

Jakob Suckale: placed disambiguation link to QRT-PCR

Jakob Suckale — Mon, 14 Jan 2008 17:27:35 GMT

placed disambiguation link to QRT-PCR

←Older revision		Revision as of 17:27, 14 January 2008
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		+	{{dablink\|[[QRT-PCR\|quantitative reverse transcription PCR]]}}
		+
	'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.		'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.

Jakob Suckale at 17:13, 14 January 2008

Jakob Suckale — Mon, 14 Jan 2008 17:13:44 GMT

←Older revision		Revision as of 17:13, 14 January 2008
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	'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.		'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.

-	Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, [[RNA extraction\|isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse ~~transcription~~ PCR ([[qRT-PCR]]) or even longer names.	+	Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, [[RNA extraction\|isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcriptase PCR ([[qRT-PCR]]) or even longer names.

	==See also==		==See also==

Jakob Suckale at 17:13, 14 January 2008

Jakob Suckale — Mon, 14 Jan 2008 17:13:26 GMT

←Older revision		Revision as of 17:13, 14 January 2008
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	'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.		'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.

-	Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, [[RNA extraction\|~~Isolated~~ RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.	+	Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, [[RNA extraction\|isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.

	==See also==		==See also==

Jakob Suckale: /* See also */

Jakob Suckale — Mon, 14 Jan 2008 17:05:52 GMT

Jakob Suckale: /* See also */

Jakob Suckale — Mon, 14 Jan 2008 17:05:34 GMT

Jakob Suckale: lead rewritten

Jakob Suckale — Mon, 14 Jan 2008 17:04:44 GMT

lead rewritten

←Older revision		Revision as of 17:04, 14 January 2008
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-	'''Quantitative PCR''' (Q-PCR or qPCR) is used to quantify starting amounts of ~~nucleic acid~~ template. ~~In quantitative PCR, the DNA amounts~~ can be measured either at the end of the PCR ~~step~~ (end-point assay) or during the PCR ~~step~~ ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.	+	'''Quantitative PCR''' (Q-PCR or qPCR) is a [[PCR techniques\|PCR technique]] used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.

-	Frequently, quantitative PCR is used to measure RNA ~~levels (although it can also be used to measure~~ DNA levels). [[RNA extraction\|Isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.	+	Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, [[RNA extraction\|Isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.

	==See also==		==See also==

Reshma P. Shetty at 20:56, 9 October 2007

Reshma P. Shetty — Tue, 09 Oct 2007 20:56:23 GMT

←Older revision		Revision as of 20:56, 9 October 2007
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	Frequently, quantitative PCR is used to measure RNA levels (although it can also be used to measure DNA levels). [[RNA extraction\|Isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.		Frequently, quantitative PCR is used to measure RNA levels (although it can also be used to measure DNA levels). [[RNA extraction\|Isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.

-	== See also ==	+	==See also==
-	* [[PCR]], [[PCR techniques]] and [[qRT-PCR]] ~~on OpenWetWare.~~	+	* [[PCR]] and [[PCR techniques]]
		+	* [[Real-time PCR]] and [[qRT-PCR\|quantitative reverse transcription PCR]]
	* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina		* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina
	* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction\|Q-PCR]]		* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction\|Q-PCR]]

Reshma P. Shetty at 20:55, 9 October 2007

Reshma P. Shetty — Tue, 09 Oct 2007 20:55:08 GMT

←Older revision		Revision as of 20:55, 9 October 2007
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-	~~#REDIRECT~~ [[Real-time PCR]]	+	'''Quantitative PCR''' (Q-PCR or qPCR) is used to quantify starting amounts of nucleic acid template. In quantitative PCR, the DNA amounts can be measured either at the end of the PCR step (end-point assay) or during the PCR step ([[Real-time PCR\|real-time PCR]]). Today [[Real-time PCR\|real-time PCR]] is more commonly used because it can be more precise.
		+
		+	Frequently, quantitative PCR is used to measure RNA levels (although it can also be used to measure DNA levels). [[RNA extraction\|Isolated RNA]] is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.
		+
		+	== See also ==
		+	* [[PCR]], [[PCR techniques]] and [[qRT-PCR]] on OpenWetWare.
		+	* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina
		+	* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction\|Q-PCR]]
		+	* The venerable [http://tech.groups.yahoo.com/group/qpcrlistserver/ qpcrlistserv]. Anyone doing qPCR should be subscribed to this list.
		+
		+	[[Category:Protocol]]
		+	[[Category:In vitro]]
		+	[[Category:DNA]]
		+	[[Category:RNA]]

←Older revision		Revision as of 17:45, 14 January 2008
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	* [[Real-time PCR]] and [[qRT-PCR\|quantitative reverse transcriptase PCR]]		* [[Real-time PCR]] and [[qRT-PCR\|quantitative reverse transcriptase PCR]]
	* [[PCR]] and [[PCR techniques]]		* [[PCR]] and [[PCR techniques]]
-	* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina
	* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction\|Q-PCR]]		* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction\|Q-PCR]]
-	* The venerable [http://tech.groups.yahoo.com/group/qpcrlistserver/ qpcrlistserv]. Anyone doing qPCR should be subscribed to this list.

	[[Category:Protocol]]		[[Category:Protocol]]

←Older revision		Revision as of 17:05, 14 January 2008
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	==See also==		==See also==
-	* [[Real-time PCR]] and [[qRT-PCR\|quantitative reverse ~~transcription~~ PCR]]	+	* [[Real-time PCR]] and [[qRT-PCR\|quantitative reverse transcriptase PCR]]
	* [[PCR]] and [[PCR techniques]]		* [[PCR]] and [[PCR techniques]]
	* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina		* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina