Q-PCR

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(See also: some links moved to QRT-PCR)
Current revision (12:29, 23 February 2009) (view source)
 
Line 1: Line 1:
 +
{{back to protocols}}
{{dablink|[[QRT-PCR|quantitative reverse transcription PCR]]}}
{{dablink|[[QRT-PCR|quantitative reverse transcription PCR]]}}

Current revision

back to protocols

Quantitative PCR (Q-PCR or qPCR) is a PCR technique used to quantify starting amounts of DNA template. Amounts can be measured either at the end of the PCR (end-point assay) or during the PCR steps (real-time PCR). Today real-time PCR is more commonly used because it can be more precise.

Frequently, quantitative PCR is used to measure RNA instead of DNA levels. To do this, isolated RNA is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcriptase PCR (qRT-PCR) or even longer names.

See also

Personal tools