Q-PCR

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(Q-PCR moved to Real-time PCR: this page focuses on real-time PCR but there is also end-point assays)
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#REDIRECT [[Real-time PCR]]
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'''Quantitative PCR''' (Q-PCR or qPCR) is used to quantify starting amounts of nucleic acid template.  In quantitative PCR, the DNA amounts can be measured either at the end of the PCR step (end-point assay) or during the PCR step ([[Real-time PCR|real-time PCR]]).  Today [[Real-time PCR|real-time PCR]] is more commonly used because it can be more precise.
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Frequently, quantitative PCR is used to measure RNA levels (although it can also be used to measure DNA levels).  [[RNA extraction|Isolated RNA]] is reverse transcribed into cDNA.  Then quantitative PCR is run on the cDNA sample.  This sub-technique is called quantitative reverse transcription PCR ([[qRT-PCR]]) or even longer names.
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== See also ==
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* [[PCR]], [[PCR techniques]] and [[qRT-PCR]] on OpenWetWare.
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* An excellent, detailed [http://pathmicro.med.sc.edu/pcr/realtime-home.htm Q-PCR tutorial] by Margaret and Richard Hunt, University of South Carolina
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* Wikipedia entry on [[wikipedia:Quantitative polymerase chain reaction|Q-PCR]]
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* The venerable [http://tech.groups.yahoo.com/group/qpcrlistserver/ qpcrlistserv]. Anyone doing qPCR should be subscribed to this list.
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[[Category:Protocol]]
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[[Category:In vitro]]
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[[Category:DNA]]
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[[Category:RNA]]

Revision as of 16:55, 9 October 2007

Quantitative PCR (Q-PCR or qPCR) is used to quantify starting amounts of nucleic acid template. In quantitative PCR, the DNA amounts can be measured either at the end of the PCR step (end-point assay) or during the PCR step (real-time PCR). Today real-time PCR is more commonly used because it can be more precise.

Frequently, quantitative PCR is used to measure RNA levels (although it can also be used to measure DNA levels). Isolated RNA is reverse transcribed into cDNA. Then quantitative PCR is run on the cDNA sample. This sub-technique is called quantitative reverse transcription PCR (qRT-PCR) or even longer names.

See also

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