Purification of DNA: Difference between revisions
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There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | |||
#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
#[[Micropure EZ and Microcon purification]] | #[[Micropure EZ and Microcon purification]] | ||
#*for small DNA fragments (~50-200 bp in length) | |||
#*removes proteins, nucleotides and salts | |||
#[[Miniprep]] | #[[Miniprep]] | ||
#*for purifying plasmid DNA from ''E. coli'' cells | |||
#[[QIAquick PCR purification]] | |||
#*for typical DNA fragments (> 200 bp in length) |
Revision as of 11:44, 31 May 2005
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
- Ethanol precipitation of small DNA fragments
- Micropure EZ and Microcon purification
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)