Purification of DNA: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(grouped protocols according to method) |
(→Alcohol precipitation: link corrected after move) |
||
| Line 2: | Line 2: | ||
===Alcohol precipitation=== | ===Alcohol precipitation=== | ||
#[[ | #[[Ethanol precipitation of nucleic acids]] | ||
#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
#[[Isopropanol Precipitation for PCR Purification]] | #[[Isopropanol Precipitation for PCR Purification]] | ||
Revision as of 03:56, 25 April 2007
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
Alcohol precipitation
- Ethanol precipitation of nucleic acids
- Ethanol precipitation of small DNA fragments
- Isopropanol Precipitation for PCR Purification
- use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns
Filtration / affinity columns
- Centrifugal filtration/Nucleic acids
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
