Purification of DNA

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#[[Ethanol precipitation of small DNA fragments]]
#[[Ethanol precipitation of small DNA fragments]]
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#[[Micropure EZ and Microcon purification]]
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#[[Centrifugal filtration/Nucleic acids]]
#*for small DNA fragments (~50-200 bp in length)
#*for small DNA fragments (~50-200 bp in length)
#*removes proteins, nucleotides and salts
#*removes proteins, nucleotides and salts

Revision as of 23:58, 13 September 2006

There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.

  1. Ethanol precipitation of small DNA fragments
  2. Centrifugal filtration/Nucleic acids
    • for small DNA fragments (~50-200 bp in length)
    • removes proteins, nucleotides and salts
  3. Miniprep
    • for purifying plasmid DNA from E. coli cells
  4. QIAquick PCR purification
    • for typical DNA fragments (> 200 bp in length)
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