Purification of DNA: Difference between revisions
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#[[QIAquick PCR purification]] | #[[QIAquick PCR purification]] | ||
#*for typical DNA fragments (> 200 bp in length) | #*for typical DNA fragments (> 200 bp in length) | ||
[[Category:Protocol]] | |||
[[Category:DNA]] | |||
[[Category:In vitro]] | |||
Revision as of 11:52, 10 July 2006
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
- Ethanol precipitation of small DNA fragments
- Micropure EZ and Microcon purification
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
