Purification of DNA
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| + | There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications. | ||
| + | |||
#[[Ethanol precipitation of small DNA fragments]] | #[[Ethanol precipitation of small DNA fragments]] | ||
#[[Micropure EZ and Microcon purification]] | #[[Micropure EZ and Microcon purification]] | ||
| + | #*for small DNA fragments (~50-200 bp in length) | ||
| + | #*removes proteins, nucleotides and salts | ||
#[[Miniprep]] | #[[Miniprep]] | ||
| + | #*for purifying plasmid DNA from ''E. coli'' cells | ||
| + | #[[QIAquick PCR purification]] | ||
| + | #*for typical DNA fragments (> 200 bp in length) | ||
Revision as of 14:44, 31 May 2005
There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.
- Ethanol precipitation of small DNA fragments
- Micropure EZ and Microcon purification
- for small DNA fragments (~50-200 bp in length)
- removes proteins, nucleotides and salts
- Miniprep
- for purifying plasmid DNA from E. coli cells
- QIAquick PCR purification
- for typical DNA fragments (> 200 bp in length)
