Purification of DNA

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(grouped protocols according to method)
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There are several methods for purifying DNA.  The one you choose depends on the nature of your DNA sample and your downstream applications.
There are several methods for purifying DNA.  The one you choose depends on the nature of your DNA sample and your downstream applications.
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===Alcohol precipitation===
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#[[Nucleic acid precipitation]]
#[[Ethanol precipitation of small DNA fragments]]
#[[Ethanol precipitation of small DNA fragments]]
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#[[Isopropanol Precipitation for PCR Purification]]
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#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns
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===Filtration / affinity columns===
#[[Centrifugal filtration/Nucleic acids]]
#[[Centrifugal filtration/Nucleic acids]]
#*for small DNA fragments (~50-200 bp in length)
#*for small DNA fragments (~50-200 bp in length)
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#[[QIAquick PCR purification]]
#[[QIAquick PCR purification]]
#*for typical DNA fragments (> 200 bp in length)
#*for typical DNA fragments (> 200 bp in length)
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#[[Isopropanol Precipitation for PCR Purification]]
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-
#*use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns
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[[Category:Protocol]]
[[Category:Protocol]]
[[Category:DNA]]
[[Category:DNA]]
[[Category:In vitro]]
[[Category:In vitro]]

Revision as of 06:32, 25 April 2007

There are several methods for purifying DNA. The one you choose depends on the nature of your DNA sample and your downstream applications.

Alcohol precipitation

  1. Nucleic acid precipitation
  2. Ethanol precipitation of small DNA fragments
  3. Isopropanol Precipitation for PCR Purification
    • use to concentrate DNA from PCR when you have verified that only a single PCR product exists. When performed properly this procedure exceeds the recovery of QIAquick PCR purification columns

Filtration / affinity columns

  1. Centrifugal filtration/Nucleic acids
    • for small DNA fragments (~50-200 bp in length)
    • removes proteins, nucleotides and salts
  2. Miniprep
    • for purifying plasmid DNA from E. coli cells
  3. QIAquick PCR purification
    • for typical DNA fragments (> 200 bp in length)
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