Protocols v.3

In vivo Escherichia coli Mesoplasma florum T7 Yeast

In vitro Nucleic acids Nucleic acid precipitation Phenol/chloroform extraction DNA Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector Polony Protocols (Church/Mitra Lab) PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi RNA In vitro transcription with T7 RNA polymerase Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNA Extraction RNase Protection Assay Protein Acrylamide gels and SDS-PAGE Coomassie Destain Coomassie Stain HPLC Silver staining Western Blot Purification of His-tagged proteins

In silico Sequence Analysis BLAST CLUSTALW Structure THREADER Homology Modelling Pathway Modelling Flux Balance Analysis Minimisation of Metabolic Adjustment Other Flow Cytometry Flow Calibration Microfluidics Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat Microscopy Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy Plate reader Perkin Elmer Victor3 -Plate reader usage protocols General resources Protocol-online- Contains a lot of useful protocols and a popular discussion section. Protocols in Development Please Contribute! Pulse-chase protein production Fixing cells B-galactosidase assay RNA Half-life Denaturing acrylamide gel purification of nucleic acids Quantification of nucleic acids