Protocols v.3

In vivo Escherichia coli Bacterial cell culture Bacterial Transformation Preparing chemically competent cells (TSS) Transforming chemically competent cells (TSS) Preparing electrocompetent cells Transforming electrocompetent cells β-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Pour LB plates Protein expression resource S_N Chemostat Protocol Mesoplasma florum Preparing culture media T7 Cesium Chloride Purification of T7 Simple lysate protocol from plaque or lysate Yeast Assaying mating Cell Cycle Arrest Dropout plates for yeast High Efficiency Transformation Layered plates Pour YPD plates Simple Yeast Lysate (for Western etc.) Time-Lapse Microscopy Yeast Colony PCR Yeast DNA Prep

In vitro Nucleic acids Nucleic acid precipitation Phenol/chloroform extraction DNA Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector Polony Protocols (Church/Mitra Lab) PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi RNA In vitro transcription with T7 RNA polymerase Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNA Extraction RNase Protection Assay Protein Acrylamide gels and SDS-PAGE Coomassie Destain Coomassie Stain HPLC Silver staining Western Blot Purification of His-tagged proteins

In silico Sequence Analysis BLAST CLUSTALW Structure THREADER Homology Modelling Pathway Modelling Flux Balance Analysis Minimisation of Metabolic Adjustment Other Flow Cytometry Flow Calibration Microfluidics Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat Microscopy Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy Plate reader Perkin Elmer Victor3 -Plate reader usage protocols General resources Protocol-online- Contains a lot of useful protocols and a popular discussion section. Protocols in Development Please Contribute! Pulse-chase protein production Fixing cells B-galactosidase assay RNA Half-life Denaturing acrylamide gel purification of nucleic acids Quantification of nucleic acids