Protocols

In vivo

Escherichia coli

Bacterial cell culture

Chemically competent cells

Electrocompetent cells

Electroporation

Miniprep

Colony PCR

Pulse-chase protein production

"Quantitative" purification of His-tagged proteins from cell lysates (analytical scale)

Yeast

High Efficiency Transformation

Yeast Colony PCR

Yeast DNA Prep

Cell Cycle Arrest

Fixing cells

B-galactosidase assay

Time-Lapse Microscopy

Simple Yeast Lysate (for Western etc.)

T7

Simple lysate protocol from plaque or lysate

Cesium Chloride Purification of T7

In vitro

Nucleic acids

Quantification of nucleic acids

Phenol/chloroform extraction

Nucleic acid precipitation

Denaturing acrylamide gel purification of nucleic acids

DNA

Agarose gel

Annealing Primers

Designing primers

DNA Ligation

Library Generation

PCR

Phosphatase treatment of linearized vector

PNK Treatment of DNA Ends

Purification of DNA

Reconstituting primers

Restriction digest

'Round-the-horn site-directed mutagenesis

Sequencing DNA

Site-directed mutagenesis

Templiphi

RNA

RNA Extraction

Northern Blot, ³²P End-Labeled Probes

Probe Prep, ³²P End-Labeled Probes

RNase Protection Assay

In vitro transcription with T7 RNA polymerase

RNA Half-life

Protein

Acrylamide gels and SDS-PAGE

Coomassie Stain

Coomassie Destain

Silver staining

Western Blot

Microfluidics

Design a Microfluidic Chip

Order a Microfluidic Chip from the Foundry

Operate Microfluidic Chemostat

Microscopy

Agarose Pads for Microscopy

Quantitative Microscopy

Flow Cytometry

Flow Calibration

Miscellaneous

Counteracting evaporation from wells in a Victor3 plate reader

Pour LB plates

Pour YPD plates

Layered plates

S_N Chemostat Protocol

beta-Galactosidase Assay (A better Miller)

10x PBS

Liquid Media