Protocols

In vivo

Escherichia coli

Bacterial cell culture

Chemically competent cells

Electrocompetent cells

Electroporation

Miniprep

Colony PCR

Pulse-chase protein production

Yeast

High Efficiency Transformation

Yeast Colony PCR

Yeast DNA Prep

Cell Cycle Arrest

Fixing cells

B-galactosidase assay

T7

Simple lysate protocol from plaque or lysate

Cesium Chloride Purification of T7

In vitro

Nucleic acids

Quantification of nucleic acids

Phenol/chloroform extraction

Nucleic acid precipitation

Denaturing acrylamide gel purification of nucleic acids

DNA

Agarose Gel

Annealing Primers

Designing Primers

DNA Ligation

Library Generation

PCR

Phosphatase treatment of linearized vector

PNK Treatment of DNA Ends

Purification of DNA

Restriction Digest

Sequencing DNA

Site-directed Mutagenesis

Templiphi

RNA

RNA Extraction

Northern Blot, ³²P End-Labeled Probes

Probe Prep, ³²P End-Labeled Probes

RNase Protection Assay

In vitro transcription with T7 RNA polymerase

RNA Half-life

Protein

Acrylamide Gels

Western Blot

Microfluidics

Design a Microfluidic Chip

Order a Microfluidic Chip from the Foundry

Operate Microfluidic Chemostat

Microscopy

Agarose Pads for Microscopy

Quantitative Microscopy

Miscellaneous

Pour plates

Endy:Victor3 counteracting evaporation in long time courses

S_N Chemostat Protocol