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=''In vivo''=
{{Navbar}}


== ''Escherichia coli'' ==
<div id="mainpage">
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[[Bacterial cell culture]]
==Contributing protocols==
===Add new protocols===
*[[Protocols/Create|'''Add a new lab- or person-specific protocol''']]
*[[Protocols/Consensus template|'''Add a consensus protocol''']] ([[Help:Consensus protocol|?]])


[[Chemically competent cells]]


[[Electrocompetent cells]]
===Improve protocols in development===
'''''Please Contribute!'''''
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Needs attention]]-->
{{#dpl:category=Needs attention|category=Protocol}}


[[Electroporation]]
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[[Miniprep]]
==''In vivo''==


[[Colony PCR]]
{{protocols group|''Acetobacter xylinum''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Acetobacter xylinum]]-->
{{#dpl:category=Acetobacter xylinum|category=Protocol|}}
}}


[[Pulse-chase protein production]]
{{protocols group|''Arabidopsis thaliana''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Arabidopsis thaliana]]-->
{{#dpl:category=Arabidopsis thaliana|category=Protocol|}}
}}


== Yeast ==
{{protocols group|''[[Escherichia coli]]''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Escherichia coli]]-->
{{#dpl:category=Escherichia coli|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Escherichia coli|category=Protocol|titlematch=%:%}}


[[High Efficiency Transformation]]
}}


[[Yeast Colony PCR]]
{{protocols group|''Lactic Acid Bacteria''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Lactobacillus]]-->
{{#dpl:category=Lactobacillus|category=Protocol|}}


[[Yeast DNA Prep]]
}}


[[Cell Cycle Arrest]]
{{protocols group|''[[Mesoplasma florum]]''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Mesoplasma florum]]-->
{{#dpl:category=Mesoplasma florum|category=Protocol|}}


[[Fixing cells]]
}}


[[B-galactosidase assay]]
{{protocols group|Mouse|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Mouse]]-->
{{#dpl:category=Mouse|category=Protocol}}
}}


== T7 ==
{{protocols group|''Mycobacterium smegmatis''|2=
[[Studier Lysate Prep|Simple lysate protocol from plaque or lysate]]
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Mycobacterium smegmatis]]-->
{{#dpl:category=Mycobacterium smegmatis|category=Protocol}}
}}


[[Cesium Chloride Purification of T7]]
{{protocols group|''[[Plasmodium falciparum]]''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Plasmodium falciparum]]-->
{{#dpl:category=Plasmodium falciparum|category=Protocol}}
}}


== Mesoplasma florum ==
{{protocols group|''[[Streptomyces:Information|Streptomyces]]''|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Streptomyces]]-->
{{#dpl:category=Streptomyces|category=Protocol}}
}}


=''In vitro''=
{{protocols group|T7|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:T7]]-->
{{#dpl:category=T7|category=Protocol}}
}}


==Nucleic acids==


[[Quantification of nucleic acids]]
{{protocols group|[[Yeast]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Yeast]]-->
{{#dpl:category=Yeast|category=Protocol}}
}}


[[Phenol/chloroform extraction]]


[[Nucleic acid precipitation]]
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<!------------------------------------------------------------------------------->
<!-----------------------------------In vitro----------------------------------->
<!------------------------------------------------------------------------------->


[[Denaturing acrylamide gel purification of nucleic acids]]
==''In vitro''==
=== DNA ===


[[Agarose Gel]]
{{protocols group|Nucleic acids|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:DNA]] and [[Category:RNA-->
{{#dpl:category=DNA|category=RNA|category=In vitro|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=DNA|category=RNA|category=In vitro|category=Protocol|titlematch=%:%}}
}}


[[Annealing Primers]]
{{protocols group|[[DNA]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:DNA]]-->
{{#dpl:category=DNA|category=In vitro|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=DNA|category=In vitro|category=Protocol|titlematch=%:%}}
}}


[[Designing Primers]]
{{protocols group|[[RNA]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:RNA]]-->
{{#dpl:category=RNA|category=In vitro|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=RNA|category=In vitro|category=Protocol|titlematch=%:%}}
}}


[[DNA Ligation]]
{{protocols group|[[Protein]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:Protein]]-->
{{#dpl:category=Protein|category=In vitro|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Protein|category=In vitro|category=Protocol|titlematch=%:%}}
}}


[[Library Generation]]
{{protocols group|[[Lipid]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:Lipid]]-->
{{#dpl:category=Lipid|category=In vitro|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Lipid|category=In vitro|category=Protocol|titlematch=%:%}}
}}


[[PCR]]
{{protocols group|Mammalian cell culture|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:Mammalian cell culture]]-->
{{#dpl:category=Mammalian cell culture|category=In vitro|category=Protocol}}
}}


[[Phosphatase treatment of linearized vector]]
{{protocols group|Plant specific protocols|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In vitro]] and [[Category:Plant]]-->
{{#dpl:category=Plant|category=In vitro|category=Protocol}}
}}
|-


[[PNK Treatment of DNA Ends]]
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<!------------------------------------------------------------------------------->
<!-----------------------------------In silico------------------------------------>
<!------------------------------------------------------------------------------->


[[Purification of DNA]]
==''In silico''==


[[Restriction Digest]]
{{protocols group|[[Cloning]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In silico]] and [[Category:Cloning]]-->
{{#dpl:category=Cloning|category=In silico|category=Protocol|titlematch=%:%}}
}}


[[Sequencing DNA]]
{{protocols group|[[Data analysis]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In silico]] and [[Category:Data analysis]]-->
{{#dpl:category=Data analysis|category=In silico|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Data analysis|category=In silico|category=Protocol|titlematch=%:%}}
}}


[[Site-directed Mutagenesis]]
{{protocols group|[[Databases]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In silico]] and [[Category:Databases]]-->
{{#dpl:category=Databases|category=In silico|category=Protocol|titlematch=%:%}}
}}


[[Templiphi]]
{{protocols group|[[Modelling]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In silico]] and [[Category:Modelling]]-->
{{#dpl:category=Modelling|category=In silico|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Modelling|category=In silico|category=Protocol|titlematch=%:%}}
}}


=== RNA ===
{{protocols group|[[Sequence analysis]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In silico]] and [[Category:Sequence analysis]]-->
{{#dpl:category=Sequence analysis|category=In silico|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Sequence analysis|category=In silico|category=Protocol|titlematch=%:%}}
}}


[[RNA Extraction]]
{{protocols group|[[Structure analysis]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:In silico]] and [[Category:Sequence analysis]]-->
{{#dpl:category=Structure|category=In silico|category=Protocol|nottitlematch=%:%}}
{{#dpl:category=Structure|category=In silico|category=Protocol|titlematch=%:%}}
}}


[[Northern Blot, 32P End-Labeled Probes]]
[[Tregwiki:Main_Page|Transcriptional Regulation]]


[[Probe Prep, 32P End-Labeled Probes]]


[[RNase Protection Assay]]
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<!------------------------------------------------------------------------------->
<!-----------------------------------OTHER--------------------------------------->
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[[In vitro transcription with T7 RNA polymerase]]
==Other==


== Protein ==
{{protocols group|General resources|2=
*[http://www.currentprotocols.com/WileyCDA/ Current Protocols] Most comprehensive source of protocols ranging from molecular biology to neuroscience.
*[http://mrw.interscience.wiley.com/emrw/9780471142720/home/ Current Protocols in Molecular Biology]
*[http://www.myjove.com/ JoVE] Journal of Visualized Experiments: An online research journal for publishing visualized (video-based) biological experiments.
*[http://protocol-online.org Protocol-online] Useful protocols and a popular discussion section.
*[http://www.springerprotocols.com Springer Protocols]
}}


[[Acrylamide Gels]]
{{protocols group|[[Aseptic Technique]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Aseptic Technique]]-->
{{#dpl:category=Aseptic Technique|category=Protocol}}
}}


[[Western Blot]]
{{protocols group|[[Flow cytometry]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Flow cytometry]]-->
{{#dpl:category=Flow cytometry|category=Protocol}}
}}


= Microfluidics =
{{protocols group|Microfluidics|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Microfluidics]]-->
{{#dpl:category=Microfluidics|category=Protocol}}
}}


[[Design a Microfluidic Chip]]
{{protocols group|[[Microscopy]]|2=
<!--To list a protocol here, "tag" the protocol page with the [[Category:Protocol]] and [[Category:Microscopy]]-->
{{#dpl:category=Microscopy|category=Protocol}}
}}


[[Order a Microfluidic Chip from the Foundry]]
{{protocols group|Plate reader|2=
*[[Endy:Victor3 plate reader|Plate reader usage protocols]]
}}


[[Operate Microfluidic Chemostat]]
{{protocols group|Miscellaneous|2=
<!--To list a protocol here, "tag" the protocol page with only [[Category:Protocol]]-->
{{#dpl:categoriesminmax=,1|category=Protocol}}
}}


= Microscopy =
{{protocols group|Need help with protocols?|2=
 
*[[Questions and Answers|Questions and Answers on OWW]]
[[Agarose Pads for Microscopy]]
}}
 
|}
[[Quantitative Microscopy]]
{{show hide all}}
 
<sitesearch>
= Miscellaneous =
title=Search Protocols
 
qualifier=protocol
[[Pour plates]]
target=
 
</sitesearch>
[[Counteracting Evaporation from 96 Well Plates in Long Time Courses on a Victor3 Plate Reader]]
</div>
 
__NOTOC__
[[S_N Chemostat Protocol]]

Revision as of 14:35, 30 July 2015

Labs & Groups
From around the world 		Courses
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Read OWW blogs

Contributing protocols

Add new protocols


Improve protocols in development

Please Contribute!

In vivo

Acetobacter xylinum

Arabidopsis thaliana

Escherichia coli

Lactic Acid Bacteria

Mesoplasma florum

Mouse

Mycobacterium smegmatis

Plasmodium falciparum

Streptomyces

T7


Yeast


In vitro

Nucleic acids

DNA

RNA

Protein

Lipid

Mammalian cell culture

Plant specific protocols

In silico

Cloning

Data analysis

Databases

Modelling

Sequence analysis

Structure analysis

Transcriptional Regulation


Other

General resources

Aseptic Technique

Flow cytometry

Microfluidics

Microscopy

Plate reader

Miscellaneous

Need help with protocols?

[show all] [hide all] <sitesearch> title=Search Protocols qualifier=protocol target= </sitesearch>

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