http://openwetware.org/index.php?title=Proteinase_K&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Thu, 23 May 2013 22:52:37 GMT http://openwetware.org/index.php?title=Proteinase_K&diff=215049&oldid=prev <p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:38, 24 June 2008</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procurement==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procurement==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx&nbsp; Qiagen]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx&nbsp; Qiagen<ins class="diffchange diffchange-inline">] or [http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas</ins>]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Use==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Use==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Usual stock solution is 20mg/ml in for example [[TBS]] &nbsp;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Usual stock solution is 20mg/ml in for example [[TBS]] &nbsp;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Stock solution can be stored at -20°C</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**Stock solution can be stored at -20°C</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Optimal temperature is 50 to 55 °C</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Can be denatured at 95 °C for 10 min</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*The recommended working concentration for Proteinase K is 0.05-1 mg/ml. </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==References==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas data sheed]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Enzyme]] [[Category:Proteases]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category:Material]] <del class="diffchange diffchange-inline">and </del>[[Category:Enzyme]] <del class="diffchange diffchange-inline">and </del>[[Category:Proteases]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr> <!-- diff generator: internal 2013-05-23 22:52:37 --> </table> Tue, 24 Jun 2008 10:38:57 GMT Torsten Waldminghaus http://openwetware.org/wiki/Talk:Proteinase_K http://openwetware.org/index.php?title=Proteinase_K&diff=194063&oldid=prev <p>New page: ==Purpose== *Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments. ==Procurement== *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accesso...</p> <p><b>New page</b></p><div>==Purpose==<br /> *Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments.<br /> <br /> ==Procurement==<br /> *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen]<br /> <br /> ==Use==<br /> *Usual stock solution is 20mg/ml in for example [[TBS]] <br /> **Stock solution can be stored at -20°C<br /> <br /> <br /> <br /> <br /> <br /> [[Category:Material]] and [[Category:Enzyme]] and [[Category:Proteases]]</div> Wed, 02 Apr 2008 16:14:24 GMT Torsten Waldminghaus http://openwetware.org/wiki/Talk:Proteinase_K