Proteinase K
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(New page: ==Purpose== *Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments. ==Procurement== *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accesso...) |
Current revision (06:38, 24 June 2008) (view source) |
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==Procurement== | ==Procurement== | ||
| - | *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen] | + | *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen] or [http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas] |
==Use== | ==Use== | ||
*Usual stock solution is 20mg/ml in for example [[TBS]] | *Usual stock solution is 20mg/ml in for example [[TBS]] | ||
**Stock solution can be stored at -20°C | **Stock solution can be stored at -20°C | ||
| + | *Optimal temperature is 50 to 55 °C | ||
| + | *Can be denatured at 95 °C for 10 min | ||
| + | *The recommended working concentration for Proteinase K is 0.05-1 mg/ml. | ||
| + | ==References== | ||
| + | [http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas data sheed] | ||
| - | + | [[Category:Material]] [[Category:Enzyme]] [[Category:Proteases]] | |
| - | + | ||
| - | [[Category:Material]] | + | |
Current revision
Contents |
Purpose
- Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments.
Procurement
Use
- Usual stock solution is 20mg/ml in for example TBS
- Stock solution can be stored at -20°C
- Optimal temperature is 50 to 55 °C
- Can be denatured at 95 °C for 10 min
- The recommended working concentration for Proteinase K is 0.05-1 mg/ml.
