Proteinase K

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(New page: ==Purpose== *Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments. ==Procurement== *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accesso...)
Current revision (06:38, 24 June 2008) (view source)
 
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==Procurement==
==Procurement==
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*Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx  Qiagen]
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*Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx  Qiagen] or [http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas]
==Use==
==Use==
*Usual stock solution is 20mg/ml in for example [[TBS]]  
*Usual stock solution is 20mg/ml in for example [[TBS]]  
**Stock solution can be stored at -20°C
**Stock solution can be stored at -20°C
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*Optimal temperature is 50 to 55 °C
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*Can be denatured at 95 °C for 10 min
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*The recommended working concentration for Proteinase K is 0.05-1 mg/ml.
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==References==
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[http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas data sheed]
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[[Category:Material]] [[Category:Enzyme]] [[Category:Proteases]]
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[[Category:Material]] and [[Category:Enzyme]] and [[Category:Proteases]]
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Current revision

Contents

Purpose

  • Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments.

Procurement

Use

  • Usual stock solution is 20mg/ml in for example TBS
    • Stock solution can be stored at -20°C
  • Optimal temperature is 50 to 55 °C
  • Can be denatured at 95 °C for 10 min
  • The recommended working concentration for Proteinase K is 0.05-1 mg/ml.

References

Fermentas data sheed

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