Proteinase K: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: ==Purpose== *Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments. ==Procurement== *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accesso...) |
No edit summary |
||
| Line 3: | Line 3: | ||
==Procurement== | ==Procurement== | ||
*Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen] | *Proteinase K can be bought at [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen] or [http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas] | ||
==Use== | ==Use== | ||
*Usual stock solution is 20mg/ml in for example [[TBS]] | *Usual stock solution is 20mg/ml in for example [[TBS]] | ||
**Stock solution can be stored at -20°C | **Stock solution can be stored at -20°C | ||
*Optimal temperature is 50 to 55 °C | |||
*Can be denatured at 95 °C for 10 min | |||
*The recommended working concentration for Proteinase K is 0.05-1 mg/ml. | |||
==References== | |||
[http://www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm Fermentas data sheed] | |||
[[Category:Material]] [[Category:Enzyme]] [[Category:Proteases]] | |||
[[Category:Material]] | |||
Latest revision as of 03:38, 24 June 2008
Purpose
- Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments.
Procurement
Use
- Usual stock solution is 20mg/ml in for example TBS
- Stock solution can be stored at -20°C
- Optimal temperature is 50 to 55 °C
- Can be denatured at 95 °C for 10 min
- The recommended working concentration for Proteinase K is 0.05-1 mg/ml.
