Protein purification tags

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Overview of tags added to protein to facilitate their purification

Contents

amino acid tags

Principle

A sequence of amino acids is added to the protein and enables the recovery of the labelled protein by its unique affinity. Usually its easiest to add the stretch of amino acids to the ends of the protein to ensure its accessibility and not to disturb the protein folding.

Examples

His tag

6+ histidine (His) residues
recovered by affinity to nickel or cobalt column

more on the His tag in the Wikipedia

FLAG tag

N-DYKDDDDK-C and others
recovered with specific antibody

- more on FLAG-tag in the Wikipedia
- protocol for purification of His-tagged protein, Kafatos lab: Kafatos:His-tagged Protein Purification (denaturing)
- protocol for purification of His-tagged protein, Wittrup lab: Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

HA tag

GST tag

fusion with the small glutathione-S-transferase (GST; 26 kDa)
recovery by affinity to substrate glutathione bound to a column, e.g. glutathione sepharose

- GST system of GE Healthcare [1]


myc tag

-

biotin - streptavidin

chemical tags

links

Wikipedia

OWW

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