Protein blot (Western) hub
From OpenWetWare
(Difference between revisions)
(→External links) |
Current revision (05:17, 27 June 2012) (view source) (→See also) |
||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
[[Image:Hub icon.png|right]] | [[Image:Hub icon.png|right]] | ||
| - | The ''' | + | The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting. |
| - | ==General | + | ==General protein blot protocol== |
| - | * [[ | + | * [[Protein blot (Western)]] |
==Western blot protocols by individual labs== | ==Western blot protocols by individual labs== | ||
| Line 17: | Line 17: | ||
==See also== | ==See also== | ||
* [[Griffin:Membrane Stripping and Reprobing]] | * [[Griffin:Membrane Stripping and Reprobing]] | ||
| + | * [[Griffin:Western Blot Optimization]] | ||
==External links== | ==External links== | ||
Current revision
The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
Contents |
General protein blot protocol
Western blot protocols by individual labs
- Endy:Tris-Tricine Acrylamide Gels
- Endy:E. coli Western Blot
- Endy: General Western Blot
- Odom:Western Blot
- Sauer:Western blot
- Ketner: Tissue culture viral lysate Western Blot
- Tissue preparation for western blot
