Protein blot (Western) hub

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(External links)
Current revision (05:17, 27 June 2012) (view source)
(See also)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
[[Image:Hub icon.png|right]]
[[Image:Hub icon.png|right]]
-
The '''Western blot''', also known as the immunoblot or the protein blot, is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
+
The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
-
==General Western blot protocol==
+
==General protein blot protocol==
-
* [[Western Blot (immunoblot)]]
+
* [[Protein blot (Western)]]
==Western blot protocols by individual labs==
==Western blot protocols by individual labs==
Line 17: Line 17:
==See also==
==See also==
* [[Griffin:Membrane Stripping and Reprobing]]
* [[Griffin:Membrane Stripping and Reprobing]]
 +
* [[Griffin:Western Blot Optimization]]
==External links==
==External links==
* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
-
* [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]
+
* [[Image:3stars.png]] [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ]
[[Category:Protocol]]  
[[Category:Protocol]]  
[[Category:In vitro]]  
[[Category:In vitro]]  
[[Category:Protein]]
[[Category:Protein]]

Current revision

The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

Contents

General protein blot protocol

Western blot protocols by individual labs

See also

External links

Personal tools