Protein blot (Western) hub
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| - | + | The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting. | |
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| - | == | + | ==General protein blot protocol== |
| + | * [[Protein blot (Western)]] | ||
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| + | ==Western blot protocols by individual labs== | ||
*[[Endy:Tris-Tricine Acrylamide Gels]] | *[[Endy:Tris-Tricine Acrylamide Gels]] | ||
*[[Endy:E. coli Western Blot]] | *[[Endy:E. coli Western Blot]] | ||
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*[[Ketner: Tissue culture viral lysate Western Blot]] | *[[Ketner: Tissue culture viral lysate Western Blot]] | ||
*[[Western Blot/Tissue Preparation|Tissue preparation for western blot]] | *[[Western Blot/Tissue Preparation|Tissue preparation for western blot]] | ||
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| + | ==See also== | ||
| + | * [[Griffin:Membrane Stripping and Reprobing]] | ||
| + | * [[Griffin:Western Blot Optimization]] | ||
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| + | ==External links== | ||
| + | * [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia] | ||
| + | * [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online] | ||
| + | * [[Image:3stars.png]] [http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ] | ||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:In vitro]] | [[Category:In vitro]] | ||
[[Category:Protein]] | [[Category:Protein]] | ||
Current revision
The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
Contents |
General protein blot protocol
Western blot protocols by individual labs
- Endy:Tris-Tricine Acrylamide Gels
- Endy:E. coli Western Blot
- Endy: General Western Blot
- Odom:Western Blot
- Sauer:Western blot
- Ketner: Tissue culture viral lysate Western Blot
- Tissue preparation for western blot
