Protein blot (Western) hub

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*[[Ketner: Tissue culture viral lysate Western Blot]]
*[[Ketner: Tissue culture viral lysate Western Blot]]
*[[Western Blot/Tissue Preparation|Tissue preparation for western blot]]
*[[Western Blot/Tissue Preparation|Tissue preparation for western blot]]
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==External links==
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* [http://en.wikipedia.org/wiki/Western_blot Western blot on Wikipedia]
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* [http://www.protocol-online.org/biology-forums/SDS-PAGE-and-Western-Blotting/ Discussion on Western blots on Protocol Online]
[[Category:Protocol]]  
[[Category:Protocol]]  
[[Category:In vitro]]  
[[Category:In vitro]]  
[[Category:Protein]]
[[Category:Protein]]

Revision as of 05:35, 15 October 2008

Overview

A Western Blot allows for the semiquantitative determination of protein expression. Crude cell lysates are loaded into a polyacrylamide gel containing a denaturing agent which give all the proteins a net negative charge. A current can then be passed through the gel and the proteins will migrate through the gel, with the largest proteins traveling the slowest, resulting in a lane where proteins become separated on the basis of their weight. The proteins can then be transferred from the gel onto a membrane (often nitrocellulose or PVDF), which can then by incubated with an antibody directed against the protein of interest. By using a detector conjugated to an antibody one can now detect specifically the protein of interest.

Relevant lab protocols

External links

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