Protein blot (Western) hub: Difference between revisions
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The ''' | The '''protein blot''', also know as '''Western blot''' or '''immunoblot''', is one of the most tried and true experiments for [[Griffin:Antibody_Basics|antibody]]-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting. | ||
==General | ==General protein blot protocol== | ||
* [[Western | * [[Protein blot (Western)]] | ||
==Western blot protocols by individual labs== | ==Western blot protocols by individual labs== | ||
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==See also== | ==See also== | ||
* [[Griffin:Membrane Stripping and Reprobing]] | * [[Griffin:Membrane Stripping and Reprobing]] | ||
* [[Griffin:Western Blot Optimization]] | |||
==External links== | ==External links== | ||
Latest revision as of 02:17, 27 June 2012
The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.
General protein blot protocol
Western blot protocols by individual labs
- Endy:Tris-Tricine Acrylamide Gels
- Endy:E. coli Western Blot
- Endy: General Western Blot
- Odom:Western Blot
- Sauer:Western blot
- Ketner: Tissue culture viral lysate Western Blot
- Tissue preparation for western blot
