Proportal: Difference between revisions

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! June 31, 2012
! June 31, 2012
| assemblies for HL III/IV single cell || Access biome.mit.edu and add them to the ProPortal.
| assemblies for HL III/IV single cell || Access biome.mit.edu and add them to the ProPortal.
  || Add your comments
  || GenBank files have been prepared for the submission of Rex's paper.
|-
|-
! June 15, 2012
! June 15, 2012
| COG cluster pipeline. || Modify the script for uploading genomes with multiple contigs, which was missing in the current pipeline.
| COG cluster pipeline. || Modify the script for uploading genomes with multiple contigs, which was missing in the current pipeline.
  || Add your comments
  || The bug in the COG pipeline has been fixed for processing genomes with multiple contigs and undefined unique protein identifiers.
|-
|-
! May 31, 2012
! May 31, 2012
| RNA-Seq processing and analysis pipeline. || Verify RNA-Seq processing pipeline. Prepare genome annotations. Run downstream analysis on phage infection RNA-Seq data.
| RNA-Seq processing and analysis pipeline. || Verify RNA-Seq processing pipeline. Prepare genome annotations. Run downstream analysis on phage infection RNA-Seq data.
  || Add your comments
  || The RNA-Seq pipeline is working as expected using BWA/DESeq/GSEA. Genome functional annotations have been investigated. A better set of parameters in Cufflinks is to be determined for Prochlorococcus genome. 
|-
|-
! February 28, 2012
! February 28, 2012
| Run COG cluster pipeline on new genomes. || The COG cluster pipeline is applied to both host and phage genomes.
| Run COG cluster pipeline on new genomes. || The COG cluster pipeline is applied to both host and phage genomes.
  || Add your comments
  || The current version of the COG scripts does not work well for genomes that have multiple contigs or whose unique protein identifiers are not defined yet.
|-
|-
|}
|}

Revision as of 07:58, 9 July 2012

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

ProPortal Prochlorococcus Portal is a web analytical tool for Prochlorococcus, a model system for Integrative Systems Biology.

The information provided here is for the general purpose of user contribution and especially collaboration. While the lab members may have access to their internal secure wiki's, however, people will benefit from sharing the information and maintaining a collaborative environment for sustainable development.

References

Project Management

For the project management in previous years, refer to Year 2011.

Schedule
Timelines Plan Status Comments
July 31, 2012 Paper for RNA-seq data analysis Finalize the data analysis. Add your comments
June 31, 2012 Paper for overlapped genes in both host and phage genomes Review the manuscript and prepare the latest version of COG clusters with overlapped genes. Add your comments
June 31, 2012 assemblies for HL III/IV single cell Access biome.mit.edu and add them to the ProPortal. GenBank files have been prepared for the submission of Rex's paper.
June 15, 2012 COG cluster pipeline. Modify the script for uploading genomes with multiple contigs, which was missing in the current pipeline. The bug in the COG pipeline has been fixed for processing genomes with multiple contigs and undefined unique protein identifiers.
May 31, 2012 RNA-Seq processing and analysis pipeline. Verify RNA-Seq processing pipeline. Prepare genome annotations. Run downstream analysis on phage infection RNA-Seq data. The RNA-Seq pipeline is working as expected using BWA/DESeq/GSEA. Genome functional annotations have been investigated. A better set of parameters in Cufflinks is to be determined for Prochlorococcus genome.
February 28, 2012 Run COG cluster pipeline on new genomes. The COG cluster pipeline is applied to both host and phage genomes. The current version of the COG scripts does not work well for genomes that have multiple contigs or whose unique protein identifiers are not defined yet.

To-do List

For the detail of current issues that have been identified, refer to the To-do List.

Proportal DB Schema

The new Proportal DB Schema is created by adding all the missing foreign keys back into the database. Some orphan records have been identified from this process and will be fixed/removed from next release of Proportal.

Release Notes

Some issues in the current version of Proportal have been resolved. Refer to the Release Notes for more details.

Strain Discussion

This section is devoted to the discussion on issues related to various strains stored in Proportal DB.

Frequently Asked Questions

This section provides answers to some frequently asked questions.