Prince:UPLC
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Common Supplies
Name | Description | Company | Catalog# | Price($) | Notes |
---|---|---|---|---|---|
Fused Silica Sleeves | adapts from 360um to 1/32" OD | VICI | C-NL.35L | 5 for $15 | |
PicoFrit Column | 100mm long, 360um OD, 75um ID, 15um tip, packed with 5um Biobasic C18 | Thermo | PFC7515-BI-10-3PK | ?? | These came in the PepFinder Kit (we're not using these right now) |
Fused Silica (25um ID; 360um OD) | general purpose fused silica | VICI | TFS36025 | $20/meter | |
Fused Silica (20um ID; 360um OD) | ultra small (ID) fused silica | Upchurch Scientific, Inc. | FS-120 | 42.40 / 2meters | |
PEEKsil | fused silica inside, PEEK outside | SGE, Inc. | [varies] | [varies] | |
Uncoated Silica Tips | Uncut silica spray tips (we can cut with cutter) | Scientific Instruments Services [New Objective makes the tips] | FS360-20-10-N-20 | $252.00/20 | |
Precut Silica Tips for ThermoFinnigan Nanospray 2 | The spray tips [5 in package] | Scientific Instruments Services [New Objective makes the tips] | FS360-25-10-N-5-CT | $135/5; $500/20 | |
Ferrule, Fingertight 1/32, for 10-32 ports | ferrules | Upchurch Scientific, Inc. | F-113 | 7.65 ea | |
Ferrule, Fingertight 360um, for 10-32 ports | ferrules | Upchurch Scientific, Inc. | F-151 | 11.70 ea | |
Nut, WINGED, Fingertight 1/16, for 10-32 ports | winged nut | Upchurch Scientific, Inc. | F-300-01 | 2.70 ea | standard nut for the system |
Nut, LONG, Fingertight 1/16, for 10-32 ports | long nut | Upchurch Scientific, Inc. | F-330-01 | 2.70 ea | standard nut for the system |
Nut, SHORT, Fingertight 1/16, for 10-32 ports | short nut | Upchurch Scientific, Inc. | F-331-01 | 2.70 ea | standard nut for the system |
Flow Rates
Table 2-3. Effective flow rate range SilicaTip emitters (from Finnigan Nanospray Ion Source Operator’s Manual 97055-97025 Revision A, October 2004)
Emitter ID (um) | Flow rate (nL/min) |
---|---|
5 | 20 to 100 |
8 | 100 to 300 |
10 | 100 to 400 |
15 | 150 to 400 |
30 | 300 to 1000 |
Table 9-1. LC/NSI/MS Parameters for Finnigan LTQ or LCQ Series mass spectrometers (Finnigan™
Nanospray Ion Source Operator’s Manual 97055-97025 Revision A October 2004)
Column Size | Flow Rate | Tip ID | Emitter Position | Capillary Temperature |
---|---|---|---|---|
50 μm | <100 to 300 nL/min | 5 to 15 μm | approx. 1 mm | 100 to 180 °C |
75 μm | 150 to 800 nL/min | 10 to 20 μm | 1 to 2 mm | 130 to 200 °C |
180 μm | 0.3 to 1.0 μL/min | 20 to 30 μm | 2 to 3 mm | 150 to 250 °C |
320 μm | 1 to 5 μL/min | 30 to 75 μm | 3 to 5 mm | 150 to 250 °C |
This is from the Thermo Chromatography Catalogue 2006-2007 (pg. 177).
ColumnID (mm) | Flow Rate Range (uL/min) | Optimum Flow Rate (ul/min) | Recommended Injection Vol. (ul) | API Source |
---|---|---|---|---|
0.32 | 4 - 10 | 5 | 0.15 | Micro-ESI |
0.18 | 1 - 3 | 2 | 0.05 | Micro-ESI |
0.1 | 0.4 - 1 | 0.5 | 0.015 | Nanospray |
0.075 | 0.2 - 0.5 | 0.3 | 0.01 | Nanospray |