Prince:BYU Lipidomics Team

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Basic Protocol

• Get sample from almost any source
• Extraction (both methods use Folch reagent [2:1 chloroform:methanol])
  • use Kaluzne method
  • trying to implement Bligh & Dyer (gold standard)
  • MTBE Extraction [1]
• Mass Spectrometry
  • ESI-Agilent TOF
  • Tandem MS-Q-Star (8-10am)
• Analysis
  • Matlab (particular toolbox?) PCA analysis

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.

Literature

Lipidomics

Lipidomics and lipid profiling in metabolomics [2]

Lipidomics: coming to grips with lipid diversity, Nature Reviews Molecular Cell Biology, 11, 593–598 (1 August 2010)

Mass Spectrometry

Practical essentials of ESI.* [3]

Solid tutorial review on mass spec "-omics."* [4]

General approaches to lipidomics. [5]

Recent advances (2011) in MS lipidomics. [6]

Basics of ESI and intrasource separation shotgun lipidomics.** [7]

• Sample preparation for intrasource separation.** [8] [9]

Multiple precursor ion and neutral loss scanning with data-dependent acquisition. [10]

Top-down lipidomic strategy.** [11]

Top-down lipidomic analysis of blood plasma.* [12]

Yeast quantitative shotgun lipidomics. [13] [14]

Bioinformatics

Chemometrics. [15] [16]

Molecular fragmentation query language. [17]

PCA package. [18]

K-OPLS package. [19]

S-plot. [20]

Special Journal Issues on Lipidomics

Journal of Chromatography B, Volume 877, Issue 26, [21]

Prostaglandins and Other Lipid Mediators, Volume 77 [22]

European Journal of Lipid Science and Technology, Volume 111, Issue 1 [23]

Frontiers in Bioscience, Volume 12, January 2007

Methods in Enzymology, Volume 432, 2007

Signalling Lipids and Lipidomics

Sphingolipid signalling. [24]

Lipid mediators in health and disease. [25]

Lipid mediators for inflammation resolution. [26]

Eicosanoid lipidomics. [27]

Bioactive lipidomics. [28]

Lipid mediator informatics-lipidomics. [29]

Mediator lipidomics. [30]

Group Members

Current

• Mary Blackburn
• Mackay Merrill
• Dean Flanders
• Landon Weist
• Rob Roden
• Greg Jackson
• Daniel Edmondson
• Matthew Linford
• Craig Thulin
• Steven Wood
• John Prince

Former or Associated

• Jennifer MacDonald
• Jonathan Lee
• Vinod Chaudhary
• Steven Herron

Relevant Publications

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1. This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.