Prince:BYU Lipidomics Team

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(Lipid Signalling)
(Literature)
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Yeast quantitative shotgun lipidomics. [http://www.pnas.org/content/106/7/2136.full] [http://www.pnas.org/content/106/7/2089.full]
Yeast quantitative shotgun lipidomics. [http://www.pnas.org/content/106/7/2136.full] [http://www.pnas.org/content/106/7/2089.full]
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Eicosanoid lipidomics. [http://www.sciencedirect.com/science/article/pii/S0003269705003386]
 
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Bioactive lipidomics. [http://pubs.acs.org/doi/abs/10.1021/ac1015563]
 
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Lipid mediator informatics-lipidomics. [http://www.springerlink.com/content/d322jt33q4j38282/]
 
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Mediator lipidomics. [http://www.sciencedirect.com/science/article/pii/S0076687907320120]
 
===Bioinformatics===
===Bioinformatics===
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Lipid mediators for inflammation resolution. [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2796.2010.02235.x/full]
Lipid mediators for inflammation resolution. [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2796.2010.02235.x/full]
 +
 +
Eicosanoid lipidomics. [http://www.sciencedirect.com/science/article/pii/S0003269705003386]
 +
 +
Bioactive lipidomics. [http://pubs.acs.org/doi/abs/10.1021/ac1015563]
 +
 +
Lipid mediator informatics-lipidomics. [http://www.springerlink.com/content/d322jt33q4j38282/]
 +
 +
Mediator lipidomics. [http://www.sciencedirect.com/science/article/pii/S0076687907320120]
==Group Members==
==Group Members==

Revision as of 16:03, 20 June 2011

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Contents

Basic Protocol

  • Get sample from almost any source
  • Extraction (both methods use Folch reagent [2:1 chloroform:methanol])
    • use Kaluzne method
    • trying to implement Bligh & Dyer (gold standard)
    • MTBE Extraction [1]
  • Mass Spectrometry
    • ESI-Agilent TOF
    • Tandem MS-Q-Star (8-10am)
  • Analysis
    • Matlab (particular toolbox?) PCA analysis

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.

Literature

Mass Spectrometry

Practical essentials of ESI.* [2]

Solid tutorial review on mass spec "-omics."* [3]

Recent advances (2011) in MS lipidomics. [4]

General aspects of lipidomics. [5]

Basics of ESI and intrasource separation shotgun lipidomics.** [6]

Tissue sample preparation for intrasource separation.** [7]

AMDMS-SL. [8]

Top-down lipidomic strategy.** [9]

Blood plasma and top-down lipidomics.* [10]

Yeast quantitative shotgun lipidomics. [11] [12]

Bioinformatics

Chemometrics. [13] [14]

S-plot. [15]

PCA package. [16]

K-OPLS package. [17]

Molecular fragmentation query language. [18]

Special Journal Issues on Lipidomics

Journal of Chromatography B, Volume 877, Issue 26, [19]

Prostaglandins and Other Lipid Mediators, Volume 77 [20]

European Journal of Lipid Science and Technology, Volume 111, Issue 1 [21]

Frontiers in Bioscience, Volume 12, January 2007

Methods in Enzymology, Volume 432, 2007

Signalling Lipids and Lipidomics

Sphingolipid signalling. [22]

Lipid mediators in health and disease. [23]

Lipid mediators for inflammation resolution. [24]

Eicosanoid lipidomics. [25]

Bioactive lipidomics. [26]

Lipid mediator informatics-lipidomics. [27]

Mediator lipidomics. [28]

Group Members

Current

  • Mary Blackburn
  • Mackay Merrill
  • Dean Flanders
  • Landon Weist
  • Rob Roden
  • Greg Jackson
  • Daniel Edmondson
  • Matthew Linford
  • Craig Thulin
  • Steven Wood
  • John Prince

Former or Associated

  • Jennifer MacDonald
  • Jonathan Lee
  • Vinod Chaudhary
  • Steven Herron

Relevant Publications

Error fetching PMID 19935646:
  1. Error fetching PMID 19935646: [P1]
    This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.

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