Prince:BYU Lipidomics Team

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(Special Journal Issues on Lipidomics)
(Literature)
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==Literature==
==Literature==
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===Lipid Signalling===
 
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Sphingolipid signalling. [http://www.nature.com/nrm/journal/v9/n2/full/nrm2329.html]
 
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Lipid mediators in health and disease. [http://www.annualreviews.org/doi/full/10.1146/annurev.pharmtox.011008.145616]
 
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Lipid mediators for inflammation resolution. [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2796.2010.02235.x/full]
 
===Mass Spectrometry===
===Mass Spectrometry===
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''Methods in Enzymology'', Volume 432, 2007
''Methods in Enzymology'', Volume 432, 2007
 +
 +
===Lipid Signalling===
 +
 +
Sphingolipid signalling. [http://www.nature.com/nrm/journal/v9/n2/full/nrm2329.html]
 +
 +
Lipid mediators in health and disease. [http://www.annualreviews.org/doi/full/10.1146/annurev.pharmtox.011008.145616]
 +
 +
Lipid mediators for inflammation resolution. [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2796.2010.02235.x/full]
==Group Members==
==Group Members==

Revision as of 16:01, 20 June 2011

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Contents

Basic Protocol

  • Get sample from almost any source
  • Extraction (both methods use Folch reagent [2:1 chloroform:methanol])
    • use Kaluzne method
    • trying to implement Bligh & Dyer (gold standard)
    • MTBE Extraction [1]
  • Mass Spectrometry
    • ESI-Agilent TOF
    • Tandem MS-Q-Star (8-10am)
  • Analysis
    • Matlab (particular toolbox?) PCA analysis

Main analytes are phosphatidylcholine and phosphatidylethanolamine. Analytes in (-) mode would be phosphatidylserines and phosphatidylethanolamines (which are expected to have stronger signal in - mode). Typical number of analytes?

FAME-GC associated with Craig Thulin and UVU.

Literature

Mass Spectrometry

Practical essentials of ESI.* [2]

Solid tutorial review on mass spec "-omics."* [3]

Recent advances (2011) in MS lipidomics. [4]

General aspects of lipidomics. [5]

Basics of ESI and intrasource separation shotgun lipidomics.** [6]

Tissue sample preparation for intrasource separation.** [7]

AMDMS-SL. [8]

Top-down lipidomic strategy.** [9]

Blood plasma and top-down lipidomics.* [10]

Yeast quantitative shotgun lipidomics. [11] [12]

Eicosanoid lipidomics. [13]

Bioactive lipidomics. [14]

Lipid mediator informatics-lipidomics. [15]

Mediator lipidomics. [16]

Bioinformatics

Chemometrics. [17] [18]

S-plot. [19]

PCA package. [20]

K-OPLS package. [21]

Molecular fragmentation query language. [22]

Special Journal Issues on Lipidomics

Journal of Chromatography B, Volume 877, Issue 26, [23]

Prostaglandins and Other Lipid Mediators, Volume 77 [24]

European Journal of Lipid Science and Technology, Volume 111, Issue 1 [25]

Frontiers in Bioscience, Volume 12, January 2007

Methods in Enzymology, Volume 432, 2007

Lipid Signalling

Sphingolipid signalling. [26]

Lipid mediators in health and disease. [27]

Lipid mediators for inflammation resolution. [28]

Group Members

Current

  • Mary Blackburn
  • Mackay Merrill
  • Dean Flanders
  • Landon Weist
  • Rob Roden
  • Greg Jackson
  • Daniel Edmondson
  • Matthew Linford
  • Craig Thulin
  • Steven Wood
  • John Prince

Former or Associated

  • Jennifer MacDonald
  • Jonathan Lee
  • Vinod Chaudhary
  • Steven Herron

Relevant Publications

Error fetching PMID 19935646:
  1. Error fetching PMID 19935646: [P1]
    This has a great protocol for negative ion mode extraction and analysis on the Orbitrap.

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