Preparing chemically competent cells

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Revision as of 09:46, 24 May 2005 by Josh K. Michener (Talk | contribs)
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  • Grow a 5-25mL culture to an OD600 of 0.2 \rightarrow 0.5
  • Centrifuge for 10 minutes at 3000rpm and 4oC.
  • Remove supernatant and replace with 10% original volume TSS.
  • Make 100uL aliquots and store at − 80oC.
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