Preparing chemically competent cells
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(Difference between revisions)
| Line 1: | Line 1: | ||
| + | *Grow a 5-25mL culture to an OD<sub>600</sub> of 0.2 <math>\rightarrow</math> 0.5 | ||
| + | *Centrifuge for 10 minutes at 3000rpm and <math>4^o</math>C. | ||
| + | *Remove supernatant and replace with 10% original volume TSS. | ||
| + | *Make 100uL aliquots and store at <math>-80^o</math>C. | ||
| + | |||
[[Category:E.Coli Protocol]] | [[Category:E.Coli Protocol]] | ||
Revision as of 09:46, 24 May 2005
- Grow a 5-25mL culture to an OD600 of 0.2
0.5
- Centrifuge for 10 minutes at 3000rpm and 4oC.
- Remove supernatant and replace with 10% original volume TSS.
- Make 100uL aliquots and store at − 80oC.
