Phusion

From OpenWetWare

Revision as of 10:32, 18 July 2005 by Ssutton (Talk | contribs)
Jump to: navigation, search

What is it?

• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Stats about Phusion™ High-Fidelity DNA Polymerase • 10x processivity compared to Taq • 50x fidelity compared to Taq • creates blunt end • error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer • non-displacing

Website: http://www.neb.com/nebecomm/products/productF-530.asp

Personal tools