Phusion
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Description
Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Statistics about Phusion™ High-Fidelity DNA Polymerase
- 10x processivity compared to Taq
- 50x fidelity compared to Taq
- Creates blunt end
- Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
- Non-displacing
Quick reference reaction mix
See the manual for details and special usage conditions.
Component | Volume for 50μl reaction | Final concentration |
---|---|---|
5x Phusion HF Buffer | 10μl | 1x |
10mM dNTPs | 1μl | 200μM each |
primer A | x μl | 0.5μM |
primer B | x μl | 0.5μM |
template DNA | xμl | |
H2O | add to 50μl | |
Phusion DNA polymerase (2U/μ) | 0.5μl | 0.02 U/pl |