Phusion: Difference between revisions
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What is it? | '''What is it?''' | ||
• a Fusion of a Pyrococcus-like enzyme | • a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity. | ||
'''Stats about Phusion™ High-Fidelity DNA Polymerase''' | |||
• 10x processivity compared to Taq | • 10x processivity compared to Taq | ||
• 50x fidelity compared to Taq | • 50x fidelity compared to Taq | ||
Revision as of 08:32, 18 July 2005
What is it?
• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Stats about Phusion™ High-Fidelity DNA Polymerase • 10x processivity compared to Taq • 50x fidelity compared to Taq • creates blunt end • error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer • non-displacing
Website: http://www.neb.com/nebecomm/products/productF-530.asp
