Phusion

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'''What is it?'''
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==Description==
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• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
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Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
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'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
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==Statistics about Phusion™ High-Fidelity DNA Polymerase==
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*10x processivity compared to Taq
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10x processivity compared to Taq
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*50x fidelity compared to Taq
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*Creates blunt end
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50x fidelity compared to Taq
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*Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
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*Non-displacing
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• creates blunt end
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• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
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• non-displacing
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'''Links'''
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==References==
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]

Revision as of 14:23, 13 September 2006

Description

Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Statistics about Phusion™ High-Fidelity DNA Polymerase

  • 10x processivity compared to Taq
  • 50x fidelity compared to Taq
  • Creates blunt end
  • Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
  • Non-displacing

References

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