Phusion

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What is it?
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'''What is it?'''
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• a Fusion of a Pyrococcus-like enzyme (Pfu) with a double-strand DNA binding domain → increased processivity.
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• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
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Stats about Phusion™ High-Fidelity DNA Polymerase:
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'''Stats about Phusion™ High-Fidelity DNA Polymerase'''
• 10x processivity compared to Taq
• 10x processivity compared to Taq
• 50x fidelity compared to Taq
• 50x fidelity compared to Taq

Revision as of 10:32, 18 July 2005

What is it?

• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Stats about Phusion™ High-Fidelity DNA Polymerase • 10x processivity compared to Taq • 50x fidelity compared to Taq • creates blunt end • error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer • non-displacing

Website: http://www.neb.com/nebecomm/products/productF-530.asp

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