Phusion

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• non-displacing
• non-displacing
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'''Website'''
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'''Links'''
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http://www.neb.com/nebecomm/products/productF-530.asp
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*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
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*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual]

Revision as of 09:29, 5 January 2006

What is it?

• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Stats about Phusion™ High-Fidelity DNA Polymerase

• 10x processivity compared to Taq

• 50x fidelity compared to Taq

• creates blunt end

• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer

• non-displacing

Links

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