Phusion: Difference between revisions
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• non-displacing | • non-displacing | ||
''' | '''Links''' | ||
http://www.neb.com/nebecomm/products/productF-530.asp | *[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | ||
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual] | |||
Revision as of 06:29, 5 January 2006
What is it?
• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Stats about Phusion™ High-Fidelity DNA Polymerase
• 10x processivity compared to Taq
• 50x fidelity compared to Taq
• creates blunt end
• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
• non-displacing
Links
