Phusion

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 16: Line 16:
'''Website'''
'''Website'''
 +
http://www.neb.com/nebecomm/products/productF-530.asp
http://www.neb.com/nebecomm/products/productF-530.asp

Revision as of 11:33, 18 July 2005

What is it?

• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Stats about Phusion™ High-Fidelity DNA Polymerase

• 10x processivity compared to Taq

• 50x fidelity compared to Taq

• creates blunt end

• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer

• non-displacing

Website

http://www.neb.com/nebecomm/products/productF-530.asp

Personal tools